Embelin reduces cutaneous TNF-α level and ameliorates skin edema in acute and chronic model of skin inflammation in mice

被引:73
作者
Kumar, G. Kalyan [1 ,2 ]
Dhamotharan, R. [2 ]
Kulkarni, Nagaraj M. [3 ]
Mahat, Mahamad Yunnus A. [3 ]
Gunasekaran, J. [2 ]
Ashfaque, Mohammad [1 ]
机构
[1] Vanta Biosci, Gummidipundi 601201, Tamil Nadu, India
[2] Presidency Coll, Postgrad Res Ctr, Dept Plant Biol & Biotechnol, Chennai, Tamil Nadu, India
[3] KLESs Coll Pharm, Postgrad Res Ctr, Dept Pharmacol, Belgaum, Karnataka, India
关键词
Embelin; Skin inflammation; TNF-alpha; Psoriasis; NECROSIS-FACTOR-ALPHA; HUMAN KERATINOCYTES; 12-O-TETRADECANOYLPHORBOL-13-ACETATE; INHIBITOR; CYTOKINES;
D O I
10.1016/j.ejphar.2011.04.037
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Tumor necrosis factor-alpha (TNF-alpha) is known to play a crucial role in the pathogenesis of psoriasis. The present study was designed to investigate the effects of embelin on lipopolysachharide induced TNF-alpha production in mice and in human keratinocytes in vitro and also to study the effect of embelin on acute and chronic skin inflammation in mice. Production of pro-inflammatory cytokines (TNF-alpha and IL-1 beta), activation of myeloperoxidase and histological assessment were examined in acute and chronic skin inflammation using 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema. Embelin inhibited topical edema in the mouse ear, leading to substantial reductions in skin thickness and tissue weight, inflammatory cytokine production, neutrophil-mediated myeloperoxidase activity, and various histopathological indicators. Furthermore, embelin was effective at reducing inflammatory damage induced by chronic TPA exposure. Our data indicate that embelin has anti-inflammatory activities in both acute and chronic irritant contact dermatitis in vivo and this effect of embelin may be due, at least in part, to the inhibition of IL-1 beta and TNF-alpha and to the subsequent blockade of leukocyte accumulation. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:63 / 69
页数:7
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