Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and Vietnam

被引：74

作者：

Dubey, J. P. ^{[1
]}

Huong, Lam Thi Thu ^{[2
]}

Lawson, B. W. L.

Subekti, D. T. ^{[3
]}

Tassi, P. ^{[4
]}

Cabaj, W. ^{[5
]}

Sundar, N. ^{[1
]}

Velmurugan, G. V. ^{[1
]}

Kwok, O. C. H. ^{[1
]}

Su, C. ^{[6
]}

机构：

[1] USDA ARS, Anim & Nat Resources Inst, Anim Parast Dis Lab, Beltsville, MD 20705 USA

[2] Univ Agr & Forestry, Fac Med Vet, Dept Parasitol & Pathobiol, Ho Chi Minh City, Vietnam

[3] Res Inst Vet Sci, Bonor 16114, Indonesia

[4] Univ Bari, Dipartimento Sanita & Benessere Anim, I-70010 Valenzano, Italy

[5] Polish Acad Sci, Witold Stefanski Inst Parasitol, PL-00818 Warsaw, Poland

[6] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA

来源：

JOURNAL OF PARASITOLOGY | 2008年 / 94卷 / 01期

关键词：

D O I：

10.1645/GE-1362.1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The prevalence of Toxoplasma gondii in free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence of the parasite's oocysts in soil because chicken feed from the ground. The prevalence of T gondii in free-range chickens from Ghana, Indonesia, Italy, Poland, and Vietnam was determined using the modified agglutination test (MAT). Antibodies to T. gondii were found in 41 (64%) of 64 chickens from Ghana, 24 (24.4%) of 98 chickens from Indonesia, 10 (12.5%) of 80 chickens from Italy, 6 (30%) of 20 chickens from Poland, and 81 (24.2%) of 330 chickens from Vietnam. Hearts and brains of chickens were bioassayed for T. gondii. Viable T. gondii was isolated from 2 chickens from Ghana, 1 chicken from Indonesia, 3 chickens from Italy, 2 chickens from Poland, and 1 chicken from Vietnam. Toxoplasma gondii isolates from 9 chickens were genotyped using 10 PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. A total of 7 genotypes was identified; the 3 isolates from chickens from Italy were clonal type 11, and the others were nonclonal. This is the first report of genetic characterization of T. gondii isolates from animals from these countries.

引用

页码：68 / 71

页数：4

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