Gold Nanoparticle-Bridge Array to Improve DNA Hybridization Efficiency of SERS Sensors

被引：38

作者：

Huang, Xiaobin ^{[1
,2
,3
]}

Zhao, Weidong ^{[2
]}

Chen, Xiangyu ^{[2
]}

Li, Jinming ^{[2
]}

Ye, Haochen ^{[2
]}

Li, Cancan ^{[2
]}

Yin, Xiaomeng ^{[2
]}

Zhou, Xinyuan ^{[2
]}

Qiao, Xuezhi ^{[2
]}

Xue, Zhenjie ^{[1
]}

Wang, Tie ^{[1
,2
,3
]}

机构：

[1] Tianjin Univ Technol, Life & Hlth Intelligent Res Inst, Tianjin Key Lab Drug Targeting & Bioimaging, Tianjin 300384, Peoples R China

[2] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China

[3] Univ Chinese Acad Sci, Sch Chem Sci, Beijing 100049, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2022年

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

MICROARRAYS; DIFFUSION; KINETICS; PROBES;

D O I：

10.1021/jacs.2c06623

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The interfacial mass transfer rate of a target has a significant impact on the sensing performance. The surface reaction forms a concentration gradient perpendicular to the surface, wherein a slow mass transfer process decreases the interfacial reaction rate. In this work, we self-assembled gold nanoparticles (AuNPs) in the gap of a SiO(2 )opal array to form a AuNP-bridge array. The diffusion paths of vertical permeability and a microvortex effect provided by the AuNP-bridge array synergistically improved the target mass transfer efficiency. As a proof of concept, we used DNA hybridization efficiency as a research model, and the surface enhanced Raman spectroscopy (SERS) signal acted as a readout index. The experimental verification and theoretical simulation show that the AuNP-bridge array exhibited rapid mass transfer and high sensitivity. The DNA hybridization efficiency of the AuNPbridge array was 15-fold higher than that of the AuNP-planar array. We believe that AuNP-bridge arrays can be potentially applied for screening drug candidates, genetic variations, and disease biomarkers.

引用

页码：17533 / 17539

页数：7

共 39 条

[1] Oriented Two-Dimensional Covalent Organic Framework Membranes with High Ion Flux and Smart Gating Nanofluidic Transport [J].