Optimising DNA binding to carbon nanotubes by non-covalent methods

被引：36

作者：

Sanz, Vanesa ^{[1
,2
]}

Borowiak, Ewa ^{[3
]}

Lukanov, Petar ^{[4
]}

Galibert, Anna Marie ^{[4
]}

Flahaut, Emmanuel ^{[4
]}

Coley, Helen M. ^{[1
]}

Silva, S. Ravi P. ^{[2
]}

McFadden, Johnjoe ^{[1
]}

机构：

[1] Univ Surrey, Fac Hlth & Med Sci, Guildford GU2 7XH, Surrey, England

[2] Univ Zaragoza, Adv Technol Inst, Nanoelect Ctr, Zaragoza 50018, Spain

[3] Tech Univ Szczecin, Ctr Knowledge Based Nanomat, PL-70322 Szczecin, Poland

[4] Univ Toulouse 3, Interuniv Res & Engn Ctr Mat CIRIMAT, F-31062 Toulouse 9, France

来源：

CARBON | 2011年 / 49卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

DELIVERY; FUNCTIONALIZATION; TRANSPORTERS; DISPERSION; SIRNA;

D O I：

10.1016/j.carbon.2010.12.064

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors. To achieve this goal, carbon nanotubes have to be functionalized to become compatible with aqueous media and to bind the genetic material. To establish the best conditions for plasmid DNA binding, we compare the dispersion properties of single-, double- and multi-walled carbon nanotubes (SWCNTs, DWCNTs and MWCNTs, respectively) functionalized with a variety of surfactants by non-covalent attachment. The DNA binding properties of the functionalized carbon nanotubes were studied and compared by electrophoresis. Furthermore, a bilayer functionalization method for DNA binding on SWCNTs was developed that utilized RNA-wrapping to solubilize the nanotubes and cationic polymers as a bridge between nanotubes and DNA. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1775 / 1781

页数：7

共 22 条

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