Amb a 1 isoforms: Unequal siblings with distinct immunological features

被引：28

作者：

Wolf, M. ^{[1
]}

Twaroch, T. E. ^{[2
]}

Huber, S. ^{[1
]}

Reithofer, M. ^{[3
]}

Steiner, M. ^{[1
,4
]}

Aglas, L. ^{[1
]}

Hauser, M. ^{[1
]}

Aloisi, I. ^{[5
]}

Asam, C. ^{[1
]}

Hofer, H. ^{[1
]}

Parigiani, M. A. ^{[1
]}

Ebner, C. ^{[6
]}

Bohle, B. ^{[3
]}

Briza, P. ^{[1
]}

Neubauer, A. ^{[2
]}

Stolz, F. ^{[2
]}

Jahn-Schmid, B. ^{[3
]}

Wallner, M. ^{[1
]}

Ferreira, F. ^{[1
]}

机构：

[1] Univ Salzburg, Dept Mol Biol, Salzburg, Austria

[2] Biomay AG, Vienna Competence Ctr, Vienna, Austria

[3] Med Univ Vienna, Dept Pathophysiol & Allergy Res, Vienna, Austria

[4] Paracelsus Med Univ, Lab Immunol & Mol Canc Res, Salzburg, Austria

[5] Univ Bologna, Dept Biol Geol & Environm Sci, Bologna, Italy

[6] Allergy Clin Reumannplatz, Vienna, Austria

来源：

ALLERGY | 2017年 / 72卷 / 12期

基金：

奥地利科学基金会;

关键词：

allergenicity; Amb a 1 allergen; cross-reactivity; immunogenicity; ragweed pollen allergy; BINDING FAB ASSAY; RAGWEED ANTIGEN-E; IMMUNOGLOBULIN G4; POLLEN ALLERGEN; ANTIBODY; IMMUNOTHERAPY; SENSITIZATION; EXPOSURE; MULTICENTER; PREVALENCE;

D O I：

10.1111/all.13196

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

BackgroundRagweed pollen represents a major allergy risk factor. Ragweed extracts contain five different isoforms of the major allergen Amb a 1. However, the immunological characteristics of Amb a 1 isoforms are not fully investigated. Here, we compared the physicochemical and immunological properties of three most important Amb a 1 isoforms. MethodsAfter purification, the isoforms were physicochemically characterized, tested for antibody binding and induction of human T-cell proliferative responses. Their immunological properties were further evaluated in vitro and in vivo in a mouse model. ResultsAmb a 1 isoforms exhibited distinct patterns of IgE binding and immunogenicity. Compared to Amb a 1.02 or 03 isoforms, Amb a 1.01 showed higher IgE-binding activity. Isoforms 01 and 03 were the most potent stimulators of patients' T cells. In a mouse model of immunization, Amb a 1.01 induced higher levels of IgG and IgE antibodies when compared to isoforms 02 and 03. Interestingly, ragweed-sensitized patients also displayed an IgG response to Amb a 1 isoforms. However, unlike therapy-induced antibodies, sensitization-induced IgG did not show IgE-blocking activity. ConclusionThe present study showed that naturally occurring isoforms of Amb a 1 possess different immunogenic and sensitizing properties. These findings should be considered when selecting sequences for molecule-based diagnosis and therapy for ragweed allergy. Due to its high IgE-binding activity, isoform Amb a 1.01 should be included in diagnostic tests. In contrast, due to their limited B- and T-cell cross-reactivity patterns, a combination of different isoforms might be a more attractive strategy for ragweed immunotherapy.

引用

页码：1874 / 1882

页数：9

共 27 条

[1] Immunoglobulin G4: an odd antibody [J].