2-Nitroimidazole-Tricarbocyanine Conjugate as a Near-Infrared Fluorescent Probe for in Vivo Imaging of Tumor Hypoxia

被引：153

作者：

Okuda, Kensuke ^{[1
]}

Okabe, Yasuyuki ^{[1
]}

Kadonosono, Tetsuya ^{[2
]}

Ueno, Takahiro ^{[1
]}

Youssif, Bahaa G. M. ^{[1
]}

Kizaka-Kondoh, Shinae ^{[2
]}

Nagasawa, Hideko ^{[1
]}

机构：

[1] Gifu Pharmaceut Univ, Lab Pharmaceut & Med Chem, Gifu 5011196, Japan

[2] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Yokohama, Kanagawa 2268501, Japan

来源：

BIOCONJUGATE CHEMISTRY | 2012年 / 23卷 / 03期

基金：

日本学术振兴会;

关键词：

SOLID TUMORS; BINDING; CANCER; PIMONIDAZOLE; REGIONS;

D O I：

10.1021/bc2004704

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a novel near-infrared (NIR) fluorescent probe, GPU-167, for in vivo imaging of tumor hypcoda. GPU-167 comprises a tricarbocyanine dye as an NIR fluorophore and two 2-nitroimidazole moieties as exogenous hypoxia markers that undergo bioreductive activation and then selective entrapment in hypoxic cells. After treatment with GPU-167, tumor cells contained significantly higher levels of fluorescence in hypoxia than in normoxia. In vivo fluorescence imaging specifically detected GPU-167 in tumors 24 h after administration. Ex vivo analysis revealed that fluorescence showed a strong correlation with hypoxia inducible factor (HIF)-1 active hypoxic regions. These data suggest that GPU-167 is a promising in vivo optical imaging probe for tumor hypoxia.

引用

页码：324 / 329

页数：6

共 27 条

[1] Detection and specific targeting of hypoxic regions within solid tumors: Current preclinical and clinical strategies
Bache, M.
Kappler, M.
Said, H. M.
Staab, A.
Vordermark, D.
[J]. CURRENT MEDICINAL CHEMISTRY, 2008, 15 (04) : 322 - 338
[2] BAKER KJ, 1966, P SOC EXP BIOL MED, V122, P957
[3] Imaging tumor hypoxia by near-infrared fluorescence tomography
Biswal, Nrusingh C.
Pavlik, Christopher
Smith, Michael B.
Aguirre, Andres
Xu, Yan
Zanganeh, Saeid
Kuhn, Liisa T.
Claffey, Kevin P.
Zhu, Quing
[J]. JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (06)
[4] Molecular Imaging of Hypoxia
Chitneni, Satish K.
Palmer, Gregory M.
Zalutsky, Michael R.
Dewhirst, Mark W.
[J]. JOURNAL OF NUCLEAR MEDICINE, 2011, 52 (02) : 165 - 168
[5] A New Prodrug-Derived Ratiometric Fluorescent Probe for Hypoxia: High Selectivity of Nitroreductase and Imaging in Tumor Cell
Cui, Lei
Zhong, Ye
Zhu, Weiping
Xu, Yufang
Du, Qingshan
Wang, Xin
Qian, Xuhong
Xiao, Yi
[J]. ORGANIC LETTERS, 2011, 13 (05) : 928 - 931
[6] Synthesis and preliminary pharmacological results on new naphthalene derivatives as 5-HT4 receptor ligands
Diouf, O
Depreux, P
Chavatte, P
Poupaert, JH
[J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2000, 35 (7-8) : 699 - 706
[7] Controlled Modulation of Serum Protein Binding and Biodistribution of Asymmetric Cyanine Dyes by Variation of the Number of Sulfonate Groups
Hamann, Franziska M.
Brehm, Robert
Pauli, Jutta
Grabolle, Markus
Frank, Wilhelm
Kaiser, Werner A.
Fischer, Dagmar
Resch-Genger, Ute
Hilger, Ingrid
[J]. MOLECULAR IMAGING, 2011, 10 (04) : 258 - 269
[8] Optical Image-guided Surgery-Where Do We Stand?
Keereweer, Stijn
Kerrebijn, Jeroen D. F.
van Driel, Pieter B. A. A.
Xie, Bangwen
Kaijzel, Eric L.
Snoeks, Thomas J. A.
Que, Ivo
Hutteman, Merlijn
van der Vorst, Joost R.
Mieog, J. Sven D.
Vahrmeijer, Alexander L.
van de Velde, Cornelis J. H.
de Jong, Robert J. Baatenburg
Lowik, Clemens W. G. M.
[J]. MOLECULAR IMAGING AND BIOLOGY, 2011, 13 (02) : 199 - 207
[9] Hypoxia-Sensitive Fluorescent Probes for in Vivo Real-Time Fluorescence Imaging of Acute Ischemia
Kiyose, Kazuki
Hanaoka, Kenjiro
Oushiki, Daihi
Nakamura, Tomomi
Kajimura, Mayumi
Suematsu, Makoto
Nishimatsu, Hiroaki
Yamane, Takehiro
Terai, Takuya
Hirata, Yasunobu
Nagano, Tetsuo
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (45) : 15846 - 15848
[10] Significance of nitroimidazole compounds and hypoxia-inducible factor-1 for imaging tumor hypoxia
Kizaka-Kondoh, Shinae
Konse-Nagasawa, Hideko
[J]. CANCER SCIENCE, 2009, 100 (08): : 1366 - 1373

← 1 2 3 →