The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

被引：553

作者：

Dheda, K

Huggett, JF ^{[1
]}

Chang, JS

Kim, LU

Bustin, SA

Johnson, MA

Rook, GAW

Zumla, A

机构：

[1] UCL Royal Free & Univ Coll Med Sch, Ctr Infect Dis & Int Hlth, London W1T 4JF, England

[2] UCL Royal Free Hosp, Dept Thorac & HIV Med, London NW3 2QG, England

[3] Barts & London Queen Marys Sch Med & Dent, Barts & London, Ctr Acad Surg, London E1 2AD, England

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 344卷 / 01期

关键词：

D O I：

10.1016/j.ab.2005.05.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：141 / 143

页数：3

共 9 条

[1] Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 29 (01) :23-39

[2]

Bustin Stephen A, 2004, J Biomol Tech, V15, P155

[3] Validation of housekeeping genes for normalizing RNA expression in real-time PCR [J].

Dheda, K ;

Huggett, JF ;

Bustin, SA ;

Johnson, MA ;

Rook, G ;

Zumla, A .

BIOTECHNIQUES, 2004, 37 (01) :112-+

[4]

Dheda K, 2004, AM J RESP CRIT CARE, V169, P1167, DOI 10.1164/ajrccm.169.10.958

[5] Real-time RT-PCR normalisation; strategies and considerations [J].

Huggett, J ;

Dheda, K ;

Bustin, S ;

Zumla, A .

GENES AND IMMUNITY, 2005, 6 (04) :279-284

[6] Counting antigen-specific T cells: A new approach for monitoring response to tuberculosis treatment? [J].

Lalvani, A .

CLINICAL INFECTIOUS DISEASES, 2004, 38 (05) :757-759

[7] Absence of a prominent Th2 cytokine response in human tuberculosis [J].

Lin, YG ;

Zhang, M ;

Hofman, FM ;

Gong, JH ;

Barnes, PF .

INFECTION AND IMMUNITY, 1996, 64 (04) :1351-1356

[8] Type 2 cytokine gene activation and its relationship to extent of disease in patients with tuberculosis [J].

Seah, GT ;

Scott, GM ;

Rook, GAW .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (01) :385-389

[9] Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [J].

Vandesompele, Jo ;

De Preter, Katleen ;

Pattyn, Filip ;

Poppe, Bruce ;

Van Roy, Nadine ;

De Paepe, Anne ;

Speleman, Frank .

GENOME BIOLOGY, 2002, 3 (07)

← 1 →