Development and Characterization of Polymorphic Genic-SSR Markers in Larix kaempferi

被引：21

作者：

Chen, Xing-Bin ^{[1
,2
]}

Xie, Yun-Hui ^{[1
,2
]}

Sun, Xiao-Mei ^{[1
,2
]}

机构：

[1] Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

[2] Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

来源：

MOLECULES | 2015年 / 20卷 / 04期

基金：

国家高技术研究发展计划(863计划);

关键词：

Larix kaempferi; unigene sequences; simple sequence repeat (SSR); polymorphism; cross-species amplification; MICROSATELLITE MARKERS; TRANSCRIPTOME;

D O I：

10.3390/molecules20046060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New simple sequence repeat (SSR) markers were developed in the Japanese larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five (1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 (90.6%) exhibited polymorphism among 24 individuals of L. kaempferi, with the number of alleles per locus (N-a), observed heterozygosity (H-o), expected heterozygosity (H-e) and polymorphic information content (PIC) averaging at 4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of cross-species amplification were observed. The genic-SSRs developed herein would be a valuable resource for genetic analysis of Larix kaempferi and related species, and also have the potential to facilitate the genetic improvement and breeding of larch.

引用

页码：6060 / 6067

页数：8

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