Orientation of the axial ligands and magnetic properties of the hemes in the cytochrome c7 family from Geobacter sulfurreducens determined by paramagnetic NMR

被引：19
作者：
Dantas, Joana M. [1 ]
Saraiva, Ivo H. [2 ]
Morgado, Leonor [1 ]
Silva, Marta A. [1 ]
Schiffer, Marianne [3 ]
Salgueiro, Carlos A. [1 ]
Louro, Ricardo O. [2 ]
机构：
[1] Univ Nova Lisboa, Fac Ciencias & Tecnol, Requimte CQFB, Dept Quim, P-2829516 Caparica, Portugal
[2] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780156 Oeiras, Portugal
[3] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA
来源：
DALTON TRANSACTIONS | 2011年 / 40卷 / 47期
关键词：
ELECTRONIC-STRUCTURE; STRUCTURAL BASIS; C(3); PROTEINS; FE(III); SHIFTS; C-13; H-1;
D O I：
10.1039/c1dt10975h
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
Geobacter sulfurreducens is a sediment bacterium that contains a large number of multiheme cytochromes. The family of five c(7) triheme periplasmic cytochromes from Geobacter sulfurreducens shows structural diversity of the heme core. Structural characterization of the relative orientation of the axial ligands of these proteins by C-13-paramagnetic NMR was carried out. The structures in solution were compared with those obtained by X-ray crystallography. For some hemes significant differences exist between the two methods such that orientation of the magnetic axes obtained from NMR data and the orientation taken from the X-ray coordinates differ. The results allowed the orientation of the magnetic axes to be defined confidently with respect to the heme frame in solution, a necessary step for the use of paramagnetic constraints to improve the complete solution structure of these proteins.
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收藏
页码：12713 / 12718
页数：6
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