Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

被引：79

作者：

Choudhury, Deepak ^{[2
,3
]}

van Noort, Danny ^{[1
]}

Iliescu, Ciprian ^{[2
]}

Zheng, Baixue ^{[1
,4
]}

Poon, Kar-Lai ^{[6
]}

Korzh, Svetlana ^{[7
]}

Korzh, Vladimir ^{[6
,7
]}

Yu, Hanry ^{[1
,2
,3
,4
,5
]}

机构：

[1] Natl Univ Singapore, MechanoBiol Inst, T Lab, Singapore 117411, Singapore

[2] ASTAR, Inst Bioengn & Nanotechnol, Singapore 138669, Singapore

[3] NUS Grad Sch Integrat Sci & Engn, CeLS, Singapore 117456, Singapore

[4] Natl Univ Singapore, Singapore MIT Alliance, Computat & Syst Biol Program, Singapore 117548, Singapore

[5] Natl Univ Singapore, Dept Physiol, Singapore 117597, Singapore

[6] Inst Mol & Cell Biol, Singapore 138673, Singapore

[7] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

LAB ON A CHIP | 2012年 / 12卷 / 05期

基金：

英国医学研究理事会;

关键词：

IN-VIVO; EMBRYONIC-DEVELOPMENT; BRACHYDANIO-RERIO; EYE DEVELOPMENT; DRUG DISCOVERY; HUMAN-DISEASE; LATERAL-LINE; DANIO-RERIO; MODEL; EXPRESSION;

D O I：

10.1039/c1lc20351g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).

引用

页码：892 / 900

页数：9

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