Aversive olfactory learning and associative long-term memory in Caenorhabditis elegans

被引:60
作者
Amano, Hisayuki [1 ]
Maruyama, Ichiro N. [1 ]
机构
[1] Okinawa Inst Sci & Technol, Informat Proc Biol Unit, Okinawa 9040412, Japan
关键词
GLUTAMATE-RECEPTOR; C-ELEGANS; PROTEIN-SYNTHESIS; INTERSTIMULUS-INTERVAL; WITHDRAWAL REFLEX; TEMPORAL DETERMINANTS; NEURONAL CONNECTIVITY; CHEMOSENSORY NEURONS; INTERTRIAL INTERVAL; SYNAPTIC PLASTICITY;
D O I
10.1101/lm.2224411
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The nematode Caenorhabditis elegans (C. elegans) adult hermaphrodite has 302 invariant neurons and is suited for cellular and molecular studies on complex behaviors including learning and memory. Here, we have developed protocols for classical conditioning of worms with 1-propanol, as a conditioned stimulus (CS), and hydrochloride (HCl) (pH 4.0), as an unconditioned stimulus (US). Before the conditioning, worms were attracted to 1-propanol and avoided HCl in chemotaxis assay. In contrast, after massed or spaced training, worms were either not attracted at all to or repelled from 1-propanol on the assay plate. The memory after the spaced training was retained for 24 h, while the memory after the massed training was no longer observable within 3 h. Worms pretreated with transcription and translation inhibitors failed to form the memory by the spaced training, whereas the memory after the massed training was not significantly affected by the inhibitors and was sensitive to cold-shock anesthesia. Therefore, the memories after the spaced and massed trainings can be classified as long-term memory (LTM) and short-term/middle-term memory (STM/MTM), respectively. Consistently, like other organisms including Aplysia, Drosophila, and mice, C. elegans mutants defective in nmr-1 encoding an NMDA receptor subunit failed to form both LTM and STM/MTM, while mutations in crh-1 encoding the CREB transcription factor affected only the LTM.
引用
收藏
页码:654 / 665
页数:12
相关论文
共 102 条
[71]   Genetic disruptions of Drosophila Pavlovian learning leave extinction learning intact [J].
Qin, H. ;
Dubnau, J. .
GENES BRAIN AND BEHAVIOR, 2010, 9 (02) :203-212
[72]   FACTORS AFFECTING HABITUATION AND RECOVERY FROM HABITUATION IN THE NEMATODE CAENORHABDITIS-ELEGANS [J].
RANKIN, CH ;
BROSTER, BS .
BEHAVIORAL NEUROSCIENCE, 1992, 106 (02) :239-249
[73]   CAENORHABDITIS-ELEGANS - A NEW MODEL SYSTEM FOR THE STUDY OF LEARNING AND MEMORY [J].
RANKIN, CH ;
BECK, CDO ;
CHIBA, CM .
BEHAVIOURAL BRAIN RESEARCH, 1990, 37 (01) :89-92
[74]   Context conditioning in habituation in the nematode Caenorhabditis elegans [J].
Rankin, CH .
BEHAVIORAL NEUROSCIENCE, 2000, 114 (03) :496-505
[75]   SIMULTANEOUS AND SUCCESSIVE ASSOCIATIONS IN SENSORY PRECONDITIONING [J].
RESCORLA, RA .
JOURNAL OF EXPERIMENTAL PSYCHOLOGY-ANIMAL BEHAVIOR PROCESSES, 1980, 6 (03) :207-216
[76]  
Riddle D., 1997, CAENORHABDITIS ELEGA, P739
[77]   Blocking memory reconsolidation reverses memory-associated changes in glutamate receptor expression [J].
Rose, Jacqueline K. ;
Rankin, Catharine H. .
JOURNAL OF NEUROSCIENCE, 2006, 26 (45) :11582-11587
[78]   Decreased sensory stimulation reduces behavioral responding, retards development, and alters neuronal connectivity in Caenorhabditis elegans [J].
Rose, JK ;
Sangha, S ;
Rai, S ;
Norman, KR ;
Rankin, CH .
JOURNAL OF NEUROSCIENCE, 2005, 25 (31) :7159-7168
[79]  
Rose JK, 2003, J NEUROSCI, V23, P9595
[80]   A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans [J].
Rose, JK ;
Kaun, KR ;
Rankin, CH .
LEARNING & MEMORY, 2002, 9 (03) :130-137