Phylogenetic Analysis of Complete rRNA Gene Sequence of Endoreticulatus sp Shengzhou from the Silkworm, Bombyx mori in Zhejiang of China

被引:0
作者
Qiu, Haihong [1 ,2 ]
Lu, Xingmeng [1 ]
Li, Mingqian [1 ]
He, Xinyi [1 ]
He, Xiangkang [1 ]
机构
[1] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Shaoyang Univ, Dept Biol & Chem Engn, Shaoyang 422000, Peoples R China
来源
JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2012年 / 11卷 / 07期
关键词
Microsporidia; Endoreticulatus sp; parasite; phylogeny; Shengzhou; China; LEPIDOPTERAN PESTS; SPACER REGION; MICROSPORIDIA; NOSEMA; SUBUNIT; DNA; ORGANIZATION; BOMBYCIDAE; COLEOPTERA; EVOLUTION;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Researchers employed a sample method directly using purified spores suspension instead of genomic DNA as PCR templete for amplifying complete rRNA genes of Endoreticulatus sp. Shengzhou, a novel microsporidian isolate from silkworm Bombyx mori collected in Shengzhou of China and studied its phyletic evolution of the isolate. The complete rRNA gene sequences of the isolate was 4,431 bp long and the arrangement of the rRNA genes was reversed as 5'-LSU-ITS-SSU-IGS-5S-3'. Morphological character and phylogenetic analysis based on the SSU rRNA gene sequence and ITS sequence indicated that Endoreticulatus sp. Shengzhou is closely related to Encloreticulatus genus.
引用
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页码:1056 / 1062
页数:7
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