Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos

被引：55

作者：

Cui, Chao ^{[1
]}

Benard, Erica L. ^{[1
]}

Kanwal, Zakia ^{[1
]}

Stockhammer, Oliver W. ^{[1
]}

van der Vaart, Michiel ^{[1
]}

Zakrzewska, Anna ^{[1
]}

Spaink, Herman P. ^{[1
]}

Meijer, Annemarie H. ^{[1
]}

机构：

[1] Leiden Univ, Inst Biol, Leiden, Netherlands

来源：

ZEBRAFISH: DISEASE MODELS AND CHEMICAL SCREENS, 3RD EDITION | 2011年 / 105卷

关键词：

PSEUDOMONAS-AERUGINOSA INFECTION; MYCOBACTERIUM-MARINUM INFECTION; MYELOID-SPECIFIC EXPRESSION; IN-SITU HYBRIDIZATION; TOLL-LIKE RECEPTOR; GENE-EXPRESSION; DEFINITIVE HEMATOPOIESIS; TRANSCRIPTOME RESPONSE; GRANULOMA-FORMATION; EARLY MACROPHAGES;

D O I：

10.1016/B978-0-12-381320-6.00012-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The major cell types of the innate immune system, macrophages and neutrophils, develop during the first two days of zebrafish embryogenesis. The interaction of these immune cells with pathogenic microbes can excellently be traced in the optically transparent zebrafish embryos. Various tools and methods have recently been developed for visualizing and isolating the zebrafish embryonic innate immune cells, for establishing infections by different micro-injection techniques, and for analyzing the host innate immune response following microbial recognition. Here we provide practical guidelines for the application of these methodologies and review the current state of the art in zebrafish infectious disease research.

引用

页码：273 / 308

页数：36

共 115 条

[1] In Vivo Analysis of Ifn-γ1 and Ifn-γ2 Signaling in Zebrafish [J].