EFFICIENT ENZYMATIC IN SITU SACCHARIFICATION OF CELLULOSE IN AQUEOUS-IONIC LIQUID MEDIA BY MICROWAVE PRETREATMENT

被引：0

作者：

Li, Lu ^{[1
,2
]}

Yu, Shi-Tao ^{[1
]}

Liu, Fu-Sheng ^{[1
]}

Xie, Cong-Xia ^{[3
]}

Xu, Cui-Zhi ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China

[2] S China Univ Technol, Pulp & Paper Engn State Key Lab, Guangzhou 510640, Guangdong, Peoples R China

[3] Qingdao Univ Sci & Technol, Key Lab Ecochem Engn, Coll Chem & Mol Engn, Minist Educ, Qingdao 266042, Peoples R China

来源：

BIORESOURCES | 2011年 / 6卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Ionic liquids; Cellulose; Enzymatic hydrolysis; DISSOLUTION; POLYSACCHARIDES; HYDROLYSIS; STRAW;

D O I：

暂无

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

Several representative ionic liquids (ILs) were synthesized, and [Emim]OAc was chosen as environment-friendly solvent for enzymatic in situ saccharification in view of its biocompatibility with both natural and microcrystalline cellulose, as well as its enzymatic activity. With the microwave pretreatment of natural and microcrystalline cellulose, directly enhancing the in situ enzymatic saccharification, the rate was compared versus an untreated control by the detection of dinitrosalicylic acid (DNS). It is suggested that the molecular structure of cellulose in the process of pretreatment was changed, e. g. intramolecular hydrogen bonds were broken (detected by FT-IR), and the crystallinity (monitored by SEM and XRD) changed significantly from a crystalline to an amorphous pattern. These changes of cellulose led to an increase of reducing sugar conversion during cellulose enzymatic hydrolysis.

引用

页码：4494 / 4504

页数：11

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