Electrochemical activity of new ferrocene-labelled PNA monomers to be applied for DNA detection: Effects of the molecular structure and of the solvent

被引：25
作者：
Baldoli, C
Licandro, E
Maiorana, S
Resemini, D
Rigamonti, C
Falciola, L
Longhi, M
Mussini, PR
机构：
[1] Univ Milan, Dept Phys Chem & Electrochem, I-20133 Milan, Italy
[2] CNR, Inst Mol Sci & Technol, I-20133 Milan, Italy
[3] Univ Milan, Ctr Excellence CISI, I-20133 Milan, Italy
[4] Univ Milan, Dept Organ & Ind Chem, I-20133 Milan, Italy
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2005年 / 585卷 / 02期
关键词：
DNA detection; PNA; ferrocene; electrochemically active markers; reversible outer-sphere electron transfer; molecular structure effect; medium effect;
D O I：
10.1016/j.jelechem.2005.08.010
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
PNA oligomers are promising candidates as DNA probes on account of their very high DNA binding power, but they generally require an appropriate electrochemically or spectroscopically active marker. This paper describes the systematic electrochemical characterization of two newly synthesized PNA monomers, derivatised with one or three active ferrocene groups, and of their precursors. Our monomers show high electrochemical activity in spite of their bulkiness; moreover, the systematicity of our investigation affords an interesting analysis of the role of the molecular structure, the degree of functionalization and the working solvent on the electrochemical activity of the organometallic site. (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：197 / 205
页数：9
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