Effects of nanoparticle-encapsulated curcumin on HIV-gp120-associated neuropathic pain induced by the P2X3 receptor in dorsal root ganglia

被引：32

作者：

Zhao, Shanhong ^{[1
,2
]}

Yang, Jinpu ^{[3
]}

Han, Xinyao ^{[4
]}

Gong, Yingxin ^{[4
]}

Rao, Shenqiang ^{[1
,2
]}

Wu, Bing ^{[1
,2
]}

Yi, Zhihua ^{[1
,2
,5
]}

Zou, Lifang ^{[1
,2
]}

Jia, Tianyu ^{[1
,2
]}

Li, Lin ^{[1
,2
]}

Yuan, Huilong ^{[1
,2
]}

Shi, Liran ^{[1
,2
]}

Zhang, Chunping ^{[2
,6
]}

Gao, Yun ^{[1
,2
]}

Li, Guilin ^{[1
,2
]}

Liu, Shuangmei ^{[1
,2
]}

Xu, Hong ^{[1
,2
]}

Liu, Hui ^{[1
,2
]}

Liang, Shangdong ^{[1
,2
]}

机构：

[1] Nanchang Univ, Med Sch, Dept Physiol, Nanchang 330006, Jiangxi, Peoples R China

[2] Jiangxi Prov Key Lab Auton Nervous Funct & Dis, Nanchang 330006, Jiangxi, Peoples R China

[3] Nanchang Univ, Med Coll, Queen Mary Sch, Nanchang 330006, Jiangxi, Peoples R China

[4] Nanchang Univ, Med Sch, Clin Dept 1, Nanchang 330006, Jiangxi, Peoples R China

[5] Nanchang Univ, Med Sch, Nursing Coll, Nanchang 330006, Jiangxi, Peoples R China

[6] Nanchang Univ, Med Sch, Dept Cell Biol, Nanchang 330006, Jiangxi, Peoples R China

来源：

BRAIN RESEARCH BULLETIN | 2017年 / 135卷

基金：

中国国家自然科学基金;

关键词：

HIV-gp120-associated neuropathic pain; P2X(3) receptor; Dorsal root ganglia; Nanoparticle-encapsulated curcumin; SENSORY NEURONS; RAT HABENULA; MECHANISMS; GP120; ATP; POLYMERIZATION; HYPERALGESIA; EXPRESSION; PUERARIN; PROTEIN;

D O I：

10.1016/j.brainresbull.2017.09.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

HIV-1 envelope glycoprotein (Glycoprotein 120, gp120) can directly stimulate primary sensory afferent neurons and cause chronic neuropathic pain. The P2X(3) receptor in the dorsal root ganglia (DRG) is associated with the transmission of neuropathic pain. Curcumin isolated from the herb Curcuma rhizome has anti-inflammatory and anti-tumor effects. The water solubility, targeting and bioavailability of curcumin can be improved by nano particle encapsulation. In this study, we sought to explore the effects of nanoparticle-encapsulated curcumin (nano curcumin) on HIV-gp120-induced neuropathic pain mediated by the P2X(3) receptor in DRG neurons. The results showed that mechanical and thermal hyperalgesia in rats treated with gp120 were increased compared to those in the control group. The expression levels of P2X(3) mRNA and protein in rats treated with gp120 were higher than those in the control group. Nano curcumin treatment decreased mechanical hyperalgesia and thermal hyperalgesia and upregulated the expression levels of P2X(3) mRNA and protein in rats treated with gp120. Nano curcumin treatment also reduced the ERK1/2 phosphorylation levels in gp120-treated rat DRG. In addition, P2X(3) agonist alpha,beta-methylene ATP (alpha,beta-meATP)-induced currents in DRG neurons cultured with gp120 significantly decreased after co-treatment with nano curcumin. Therefore, nano curcumin treatment may inhibit P2X(3) activation, decrease the sensitizing DRG primary afferents and relieve mechanical hyperalgesia and thermal hyperalgesia in gp120-treated rats.

引用

页码：53 / 61

页数：9

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