In vitro plant regeneration and micropropagation of Liriope platyphylla

A rapid protocol for efficient shoot organogenesis and plant regeneration from meristem cultures of Liriope platyphylla Wang et Tang was developed. Meristem explants were cultured with different concentration of cytokinins. After that, 1 mg/L of each cytokinin was supplemented with various concentrations of auxins to determine the potential regeneration capacity and growth of shoots from excised meristem culture. Among the cytokinins, 6-benzylaminopurine and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea showed the highest efficiency for shoot initiation, whereas kinetin and zeatin showed higher efficacy for shoot growth after 6 weeks in culture. In this study, zeatin (with any auxin) was highly effective for the initiation of shoot. Among the cytokinin/auxins combinations, zeatin with 0.1 mg/L indole-3-acetic acid proved optimal for yielding the highest number of shoots (4.2 per explant), followed by zeatin with 1 mg/L indole-3-butyric acid and zeatin with 0.1 mg/L naphthalene acetic acid. The combined application of zeatin and auxins might play a vital role in shoot organogenesis of Liriope platyphylla.