Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes

被引：198

作者：

König, K ^{[1
]}

Becker, TW ^{[1
]}

Fischer, P ^{[1
]}

Riemann, I ^{[1
]}

Halbhuber, KJ ^{[1
]}

机构：

[1] Univ Jena, Inst Anat 2, D-07743 Jena, Germany

来源：

OPTICS LETTERS | 1999年 / 24卷 / 02期

关键词：

D O I：

10.1364/OL.24.000113

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The influence of the pulse length, tau, of ultrashort laser pulses at 780 and 920 nm on cell vitality and cellular reproduction has been studied. A total of 2400 nonlabeled cells were exposed to a highly focused scanning beam from a mode-locked 80-MHz Ti:sapphire laser with 60-mu s pixel dwell time. For the same pulse energy, destructive effects were more pronounced for shorter pulses. The damage behavior was found to follow approximately a P-2/tau dependence (P, mean power), indicating that cell destruction is likely based on a two-photon excitation process rather than a one- or a three-photon event. Therefore, femtosecond as well as picosecond pulses provide approximately the same relative optical window for safe two-photon fluorescence microscopy. (C) 1999 Optical Society of America.

引用

页码：113 / 115

页数：3

共 17 条

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[2]

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