Effects of concentration and vendor specific composition of formalin on postmortem MRI of the human brain

被引：20

作者：

Birkl, Christoph ^{[1
]}

Soellradl, Martin ^{[1
]}

Toeglhofer, Anna Maria ^{[2
]}

Krassnig, Stefanie ^{[2
]}

Leoni, Marlene ^{[2
]}

Pirpamer, Lukas ^{[1
]}

Vorauer, Thomas ^{[3
]}

Krenn, Heinz ^{[3
]}

Haybaeck, Johannes ^{[2
,4
]}

Fazekas, Franz ^{[1
]}

Ropele, Stefan ^{[1
]}

Langkammer, Christian ^{[1
]}

机构：

[1] Med Univ Graz, Dept Neurol, Auenbruggerpl 22, A-8036 Graz, Austria

[2] Med Univ Graz, Inst Pathol, Dept Neuropathol, Graz, Austria

[3] Karl Franzens Univ Graz, Inst Phys, Expt Phys, Graz, Austria

[4] Otto von Guericke Univ, Dept Pathol, Fac Med, Magdeburg, Germany

来源：

MAGNETIC RESONANCE IN MEDICINE | 2018年 / 79卷 / 02期

基金：

奥地利科学基金会;

关键词：

formalin fixation; formalin concentration; postmortem MRI; formalin fixed brain tissue; relaxation properties of formalin; magnetic properties of formalin; FORMALDEHYDE CROSS-LINKING; RELAXATION-TIMES; FIXATION; TEMPERATURE; TISSUE; ALTERS; T-2; T2;

D O I：

10.1002/mrm.26699

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

PurposeFormalin fixation prevents tissue autolysis by crosslinking proteins and changes tissue microstructure and MRI signal characteristics. Previous studies showed high variations in MR relaxation time constants of formalin fixed brain tissue, which has been attributed to the use of different formalin concentrations. Our investigations confirmed the influence of formalin concentration on relaxation times and unexpectedly revealed an influence of vendor specific formalin composition, which has not been investigated so far. MethodsWe systematically analyzed relaxation times of human brain tissue fixed with 4% and 10% formalin compared with unfixed condition at 3 Tesla MRI. Furthermore, we assessed relaxation times of nine formalin solutions from different vendors and performed comparisons of their magnetic susceptibility by SQUID (superconducting quantum interference device) magnetometry. ResultsTissue relaxation times decreased approximately twice as fast using 10% than in 4% formalin fixation. The vendor specific composition of the formalin solutions and concentration dependent paramagnetic effects showed a substantial contribution to differences in relaxation times of formalin. ConclusionOur study demonstrates that differences of the formalin composition have substantial effects on MRI signal characteristics after fixation, which can explain the divergence of reported relaxation times beyond the effect of differences in formalin concentration. Magn Reson Med 79:1111-1115, 2018. (c) 2017 International Society for Magnetic Resonance in Medicine.

引用

页码：1111 / 1115

页数：5

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