ATR-IR spectroscopy as applied to nucleic acid films

被引:7
|
作者
Stepanyugin, AV [1 ]
Samijlenko, SP [1 ]
Martynenko, OI [1 ]
Hovorun, DM [1 ]
机构
[1] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, UA-03143 Kiev, Ukraine
关键词
ATR-TR spectroscopy; DNA; RNA; dry films; cleaning procedure; ATA;
D O I
10.1016/j.saa.2004.09.019
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
For the first time the ATR technique was applied to obtain IR absorption spectra of DNA and RNA dry films. There was worked out procedure of the nucleic acid removal from germanium plate, which obviously was a main obstacle to application of ATR-IR spectroscopy to nucleic acids. This technique of IR spectroscopy was applied to confirmation of RNA tropism of aurin tricarboxylic acid observed by molecular biological methods. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:2267 / 2269
页数:3
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