Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate

被引：35

作者：

Kim, Young-Hoon ^{[2
]}

Kim, Sung Hoon ^{[1
]}

Lee, Hoi Woul ^{[1
]}

Chae, Hee Dong ^{[1
]}

Kim, Chung-Hoon ^{[1
]}

Kang, Byung Moon ^{[1
]}

机构：

[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Obstet & Gynecol, Seoul 138736, South Korea

[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Pharmacol, Seoul 138736, South Korea

来源：

FERTILITY AND STERILITY | 2010年 / 94卷 / 06期

关键词：

Phthalate; endocrine disruptor; endometrium; endometriosis; pathogenesis; HIGH PLASMA-CONCENTRATIONS; INCREASED EXPRESSION; WOMEN; ESTERS;

D O I：

10.1016/j.fertnstert.2010.04.027

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay, fluorescent activated cell sorter analysis, and microscopic evaluation after Hoechst staining, we revealed that in vitro treatment with DEHP leads to increased viability of Ishikawa cells as well as endometrial stromal cells in serum-free condition and following exposure to hydrogen peroxide, which suggests that exposure to phthalate might play a role in the establishment of endometriosis. (Fertil Steril (R) 2010;94:2413-6. (C)2010 by American Society for Reproductive Medicine.)

引用

页码：2413 / 2416

页数：4

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