A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo

被引：125
作者：
Couvillion, Mary T. [1 ]
Sachidanandam, Ravi [2 ]
Collins, Kathleen [1 ]
机构：
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Mt Sinai Sch Med, Dept Genet & Genom Sci, New York, NY 10029 USA
来源：
GENES & DEVELOPMENT | 2010年 / 24卷 / 24期
关键词：
Argonaute/Piwi RNP; tRNA cleavage; small RNA; Tetrahymena thermophila; DICER-LIKE PROTEIN; THERMOPHILA; BIOGENESIS; EXPRESSION; CLEAVAGE; SIRNAS; STRESS; SLICER;
D O I：
10.1101/gad.1996210
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Argonaute/Piwi proteins associate with small RNAs that typically provide sequence specificity for RNP function in gene and genome regulation. Here we show that Twi12, a Tetrahymena Piwi protein essential for growth, is loaded with mature tRNA fragments. The tightly bound similar to 18- to 22-nucleotide tRNA 3' fragments are biochemically distinct from the tRNA halves produced transiently in response to stress. Notably, the end positions of Twi12-bound tRNA 3' fragments precisely match RNAs detected in total small RNA of mouse embryonic stem cells and human cancer cells. Our studies demonstrate unanticipated evolutionary conservation of mature tRNA processing to tRNA fragment small RNAs.
引用
收藏
页码：2742 / 2747
页数：6
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