A 5.8S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution

被引:57
作者
Cullings, KW
Vogler, DR
机构
[1] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA
[2] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA
关键词
5.8S rRNA; DNA database; DNA sequencing PCR;
D O I
10.1046/j.1365-294x.1998.00409.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We compiled a 5.8S nuclear ribosomal gene sequence database for animals, plants, and fungi using both newly generated and GenBank sequences. We demonstrate the utility of this database as an internal check to determine whether the target organism and not a contaminant has been sequenced, as a diagnostic tool for ecologists and evolutionary biologists to determine the placement of asexual fungi within larger taxonomic groups, and as a tool to help identify fungi that form ectomycorrhizae.
引用
收藏
页码:919 / 923
页数:5
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