Supplementation with Angelica keiskei inhibits expression of inflammatory mediators in the gastric mucosa of Helicobacter pylori-infected mice

Oxidative stress is involved in the pathogenesis of Helicobacter pylori associated gastric ulceration and carcinogenesis. The oxidant-sensitive transcription factor, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B), regulates expression of inflammatory mediators such as interferon gamma (IFN-gamma), cyclooxygenase 2 (COX -2), and inducible nitric oxide synthase (iNOS). These inflammatory mediators increased in gastric mucosal tissues from patients infected with H pylori. Angelica keiskei (AK), a green leafy vegetable, is rich in carotenoids and flavonoids and shows antioxidant and anti-inflammatory activities. Therefore, we hypothesized that AK may protect the gastric mucosa of H pylori infected mice against inflammation. We determined lipid peroxide abundance, myeloperoxidase activity, expression levels of inflammatory mediators (IFN-gamma, COX-2, and iNOS), NF-kappa B-DNA binding activity, and histologic changes in gastric mucosal tissues. The antioxidant N-acetylcysteine served as the positive control treatment. Supplementation with AK suppressed increases in lipid peroxide abundance, myeloperoxidase activity, induction of inflammatory mediators (IFN-gamma, COX -2, and iNOS), activation of NF-kappa B, and degradation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor a in gastric mucosal tissue from H pylori infected mice. Inhibition of H pylori induced alterations by AK was similar to that by N-acetylcysteine. Taken together, these results suggest that supplementation with AK may prevent H pylori induced gastric inflammation by inhibiting NF-kappa B mediated induction of inflammatory mediators in the gastric mucosa of patients infected with H pylori. (C) 2016 Elsevier Inc. All rights reserved.