Tunable light and drug induced depletion of target proteins

被引：31

作者：

Deng, Wen ^{[1
]}

Bates, Jack A. ^{[1
]}

Wei, Hai ^{[1
]}

Bartoschek, Michael D. ^{[1
]}

Conradt, Barbara ^{[1
]}

Leonhardt, Heinrich ^{[1
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Dept Biol 2, Munich, Germany

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

关键词：

PROGRAMMED CELL-DEATH; DNA-LIGASE-I; LIVING CELLS; LIVE CELLS; DEGRADATION; STABILITY; UBIQUITINATION; DIMERIZATION; SPECIFICITY; EXPRESSION;

D O I：

10.1038/s41467-019-14160-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks.

引用

页数：13

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