Quantifying post-transcriptional regulation in the development of Drosophila melanogaster

被引：63

作者：

Becker, Kolja ^{[1
]}

Bluhm, Alina ^{[1
]}

Casas-Vila, Nuria ^{[1
]}

Dinges, Nadja ^{[1
]}

Dejung, Mario ^{[1
]}

Sayols, Sergi ^{[1
]}

Kreutz, Clemens ^{[2
]}

Roignant, Jean-Yves ^{[1
]}

Butter, Falk ^{[1
]}

Legewie, Stefan ^{[1
]}

机构：

[1] IMB, Ackermannweg 4, D-55128 Mainz, Germany

[2] Univ Freiburg, Ctr Biosyst Anal ZBSA, Habsburger Str 49, D-79104 Freiburg, Germany

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

YEAST SACCHAROMYCES-CEREVISIAE; TO-ZYGOTIC TRANSITION; GENOME-WIDE ANALYSIS; MESSENGER-RNA; EXPRESSION REGULATION; PROTEIN STABILITY; IN-VIVO; BINDING; TRANSLATION; QUANTIFICATION;

D O I：

10.1038/s41467-018-07455-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Even though proteins are produced from mRNA, the correlation between mRNA levels and protein abundances is moderate in most studies, occasionally attributed to complex post-transcriptional regulation. To address this, we generate a paired transcriptome/proteome time course dataset with 14 time points during Drosophila embryogenesis. Despite a limited mRNA-protein correlation (rho = 0.54), mathematical models describing protein translation and degradation explain 84% of protein time-courses based on the measured mRNA dynamics without assuming complex post transcriptional regulation, and allow for classification of most proteins into four distinct regulatory scenarios. By performing an in-depth characterization of the putatively post-transcriptionally regulated genes, we postulate that the RNA-binding protein Hrb98DE is involved in post-transcriptional control of sugar metabolism in early embryogenesis and partially validate this hypothesis using Hrb98DE knockdown. In summary, we present a systems biology framework for the identification of post-transcriptional gene regulation from large-scale, time-resolved transcriptome and pro-teome data.

引用

页数：14

共 65 条

[1] Global signatures of protein and mRNA expression levels [J].