A Neuronal piRNA Pathway Inhibits Axon Regeneration in &ITC&IT. &ITelegans&IT

被引：53
作者：
Kim, Kyung Won [1 ]
Tang, Ngang Heok [1 ]
Andrusiak, Matthew G. [1 ]
Wu, Zilu [1 ]
Chisholm, Andrew D. [1 ]
Jin, Yishi [1 ,2 ]
机构：
[1] Univ Calif San Diego, Div Biol Sci, Sect Neurobiol, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Sch Med, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
来源：
NEURON | 2018年 / 97卷 / 03期
关键词：
CAENORHABDITIS-ELEGANS; C; ELEGANS; SLICER ACTIVITY; EPIGENETIC MEMORY; CRYSTAL-STRUCTURE; NERVOUS-SYSTEM; NONCODING RNAS; PIWI; GERMLINE; BIOGENESIS;
D O I：
10.1016/j.neuron.2018.01.014
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The PIWI-interacting RNA (piRNA) pathway has long been thought to function solely in the germline, but evidence for its functions in somatic cells is emerging. Here we report an unexpected role for the piRNA pathway in Caenorhabditis elegans sensory axon regeneration after injury. Loss of function in a subset of components of the piRNA pathway results in enhanced axon regrowth. Two essential piRNA factors, PRDE-1 and PRG-1/PIWI, inhibit axon regeneration in a gonad-independent and cell-autonomous manner. By smFISH analysis we find that prde-1 transcripts are present in neurons, as well as germ cells. The piRNA pathway inhibits axon regrowth independent of nuclear transcriptional silencing but dependent on the slicer domain of PRG-1/PIWI, suggesting that post-transcriptional gene silencing is involved. Our results reveal the neuronal piRNA pathway as a novel intrinsic repressor of axon regeneration.
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收藏
页码：511 / +
页数：15
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