RNA Interference in Protozoan Parasites: Achievements and Challenges

被引：99

作者：

Kolev, Nikolay G. ^{[3
]}

Tschudi, Christian ^{[3
]}

Ullu, Elisabetta ^{[1
,2
]}

机构：

[1] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06536 USA

[2] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06536 USA

[3] Yale Univ, Sch Publ Hlth, Div Epidemiol Microbial Dis, New Haven, CT 06536 USA

来源：

EUKARYOTIC CELL | 2011年 / 10卷 / 09期

基金：

美国国家卫生研究院;

关键词：

DOUBLE-STRANDED-RNA; EUKARYOTE TRYPANOSOMA-BRUCEI; SINGLE ARGONAUTE PROTEIN; SMALL SILENCING RNAS; TOXOPLASMA-GONDII; ENTAMOEBA-HISTOLYTICA; GENE-EXPRESSION; CAENORHABDITIS-ELEGANS; PLASMODIUM-FALCIPARUM; GIARDIA-INTESTINALIS;

D O I：

10.1128/EC.05114-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Protozoan parasites that profoundly affect mankind represent an exceptionally diverse group of organisms, including Plasmodium, Toxoplasma, Entamoeba, Giardia, trypanosomes, and Leishmania. Despite the overwhelming impact of these parasites, there remain many aspects to be discovered about mechanisms of pathogenesis and how these organisms survive in the host. Combined with the ever-increasing availability of sequenced genomes, RNA interference (RNAi), discovered a mere 13 years ago, has enormously facilitated the analysis of gene function, especially in organisms that are not amenable to classical genetic approaches. Here we review the current status of RNAi in studies of parasitic protozoa, with special emphasis on its use as a postgenomic tool.

引用

页码：1156 / 1163

页数：8

共 78 条

[11] Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites [J].

Baum, Jake ;

Papenfuss, Anthony T. ;

Mair, Gunnar R. ;

Janse, Chris J. ;

Vlachou, Dina ;

Waters, Andrew P. ;

Cowman, Alan F. ;

Crabb, Brendan S. ;

de Koning-Ward, Tania F. .

NUCLEIC ACIDS RESEARCH, 2009, 37 (11) :3788-3798

[12] Protozomics: Trypanosomatid parasite genetics comes of age [J].

Beverley, SM .

NATURE REVIEWS GENETICS, 2003, 4 (01) :11-19

[13] The art and design of genetic screens: RNA interference [J].

Boutros, Michael ;

Ahringer, Julie .

NATURE REVIEWS GENETICS, 2008, 9 (07) :554-566

[14] A Complex Small RNA Repertoire Is Generated by a Plant/Fungal-Like Machinery and Effected by a Metazoan-Like Argonaute in the Single-Cell Human Parasite Toxoplasma gondii [J].

Braun, Laurence ;

Cannella, Dominique ;

Ortet, Philippe ;

Barakat, Mohamed ;

Sautel, Celine F. ;

Kieffer, Sylvie ;

Garin, Jerome ;

Bastien, Olivier ;

Voinnet, Olivier ;

Hakimi, Mohamed-Ali .

PLOS PATHOGENS, 2010, 6 (05) :1-20

[15] Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters [J].

Burkard, Gabriela Schumann ;

Jutzi, Pascal ;

Roditi, Isabel .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2011, 175 (01) :91-94

[16] High Throughput Genome-Wide Survey of Small RNAs from the Parasitic Protists Giardia intestinalis and Trichomonas vaginalis [J].

Chen, Xiaowei ;

Collins, Lesley J. ;

Biggs, Patrick J. ;

Penny, David .

GENOME BIOLOGY AND EVOLUTION, 2009, 1 :165-175

[17] Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi [J].

DaRocha, WD ;

Otsu, K ;

Teixeira, SMR ;

Donelson, JE .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2004, 133 (02) :175-186

[18]

Djikeng A, 2001, RNA, V7, P1522

[19] The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNA interference library [J].

Drew, ME ;

Morris, JC ;

Wang, ZF ;

Wells, L ;

Sanchez, M ;

Landfear, SM ;

Englund, PT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (47) :46596-46600

[20] RNAi in Budding Yeast [J].

Drinnenberg, Ines A. ;

Weinberg, David E. ;

Xie, Kathleen T. ;

Mower, Jeffrey P. ;

Wolfe, Kenneth H. ;

Fink, Gerald R. ;

Bartel, David P. .

SCIENCE, 2009, 326 (5952) :544-550

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