An automated microfluidic platform for calcium imaging of chemosensory neurons in Caenorhabditis elegans

被引:60
作者
Chokshi, Trushal Vijaykumar [2 ]
Bazopoulou, Daphne [1 ]
Chronis, Nikos [1 ,3 ]
机构
[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Elect & Comp Engn, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
关键词
BEHAVIOR; SYSTEM;
D O I
10.1039/c004658b
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Functional fluorescence imaging methods are widely used to study cellular physiology. When applied to small organisms, these methods suffer from low-throughput due to the laborious immobilization/stimulus delivery procedure that is typically involved during imaging. Here, we describe the development of an automated microfluidic-based platform for performing automated neuronal functional (calcium) imaging in the roundworm Caenorhabditis elegans. The platform, capable of processing tens to hundreds of worms per hour, immobilizes individual worms, delivers a chemical odor to their nose and collects calcium imaging data from single neurons without any manual intervention. We used the developed platform to obtain a large number of calcium responses from worms of different ages (212 worms were imaged in total). The calcium imaging data revealed significant difference in the responses from young and old worms, indicating that neural functionality is age-dependent. We believe that such a technology will be an essential tool for obtaining repeatable and accurate functional imaging data from a large population of worms, in order to minimize stochastic biological noise and identify statistically significant trends.
引用
收藏
页码:2758 / 2763
页数:6
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