Embryotoxicity assay of aflatoxin produced by Aspergillus parasiticus NRRL 2999

被引:40
作者
Celik, I [1 ]
Oguz, H
Demet, Ö
Boydak, M
Dönmez, HH
Sur, E
Nizamlioglu, F
机构
[1] Selcuk Univ, Dept Histol & Embryol, Fac Vet Med, TR-42031 Konya, Turkey
[2] Yuzuncu Yil Univ, Van, Turkey
[3] Minist Agr, Vet Control & Res Inst, Konya, Turkey
关键词
D O I
10.1080/713654961
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
1. The embryotoxicity of mixed aflatoxins (AF) and aflatoxin B-1 (AFB(1)) were evaluated by a modified chick embryotoxicity screening test (CHEST). Adverse effects on the early embryonic development of thymus and bursa of Fabricius were also investigated by light microscopy. AF consisted of 83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1) and 1.12% AFG(2). 2. A total of 448 fertilised laying hens' eggs were used. AF and AFB(1) were injected into the eggs at doses of 10, 100 and 1000 ng/egg. Embryonic developmental stages were evaluated according to the Hamburger-Hamilton scale (HH-scale). 3. The results showed that AFB(1) given at 10 ng/egg had a significantly (P<0.05) greater embryotoxic effect than AF given at a similar dose. The higher doses of both AF and AFB(1) caused higher embryonic mortality and also an increase in early deaths. 4. In the groups receiving 100 ng/egg AF and AFB(1) an abnormal development was seen, with a protruded central region, corresponding to the area pellucida of the blastoderm. No other developmental abnormality attributable to AF or AFB(1) was found.
引用
收藏
页码:401 / 409
页数:9
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