A virus-MIPs fluorescent sensor based on FRET for highly sensitive detection of JEV

被引：41

作者：

Liang, Caishuang ^{[1
]}

Wang, Huan ^{[1
]}

He, Kui ^{[1
]}

Chen, Chunyan ^{[1
]}

Chen, Xiaoming ^{[1
]}

Gong, Hang ^{[1
]}

Cai, Changqun ^{[1
]}

机构：

[1] Xiangtan Univ, Key Lab Environm Friendly Chem & Applicat, Minist Educ, Coll Chem, Xiangtan 411105, Hunan, Peoples R China

来源：

TALANTA | 2016年 / 160卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent sensor; Virus-molecular imprinted polymers (virus-mips); Fluorescence resonance energy transfer (fret); Pyrene-1-carboxaldehyde (pc); Japanese encephalitis virus (jev); MOLECULARLY IMPRINTED POLYMERS; PHAGE DISPLAY; RECOGNITION; SURFACE; SELECTION; QUANTIFICATION; ENHANCEMENT; PARTICLES; STABILITY; BINDING;

D O I：

10.1016/j.talanta.2016.06.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Major stumbling blocks in the recognition and detection of virus are the unstable biological recognition element or the complex detection means. Here a fluorescent sensor based on virus-molecular imprinted polymers (virus-MIPs) was designed for specific recognition and highly sensitive detection of Japanese encephalitis virus (JEV). The virus-MIPs were anchored on the surface of silica microspheres modified by fluorescent dye, pyrene-1-carboxaldehyde (PC). The fluorescence intensity of PC can be enhanced by the principle of fluorescence resonance energy transfer (FRET), where virus acted as energy donor and PC acted as energy acceptor. The enhanced fluorescence intensity was proportional to the concentration of virus in the range of 24-960 pM, with a limit of detection (LOD, 3 sigma) of 9.6 pM, and the relative standard deviation was 1.99%. In additional, the specificity study confirmed the resultant MIPs has high-selectivity for JEV. This sensor would become a new key for the detection of virus because of its high sensitive, simple operation, high stability and low cost. (C) 2016 Published by Elsevier B.V.

引用

页码：360 / 366

页数：7

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