Electrochemical genosensor based on three-dimensional DNA polymer brushes monolayers

被引：9

作者：

Henry, Olivier Y. F. ^{[1
]}

Kirwan, Sinead ^{[1
]}

Mehdi Debela, Ahmed ^{[1
]}

O'Sullivan, Ciara K. ^{[1
,2
]}

机构：

[1] Univ Rovira & Virgili, Dept Engn Quim, Nanobiotechnol & Bioanal Grp, Tarragona 43007, Spain

[2] Passeig Lluis Co 23, Inst Catalana Recerca & Estudis Avancats, Barcelona 08010, Spain

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2011年 / 13卷 / 11期

关键词：

Polymer brushes; Atom transfer polymerisation; DNA;

D O I：

10.1016/j.elecom.2011.09.010

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A new approach to the three dimensional integration of short DNA strands at gold electrode surfaces via the in situ formation of DNA-acrylamide conjugates is presented. Surface initiated atom transfer radical polymerisation was employed to grow acrylamide brushes co-polymerised in the presence of acrylamide modified DNA probes. This strategy was demonstrated for the realisation of biofunctionalised thin polymer films capable of binding its complementary 105-base DNA amplicon. The synthesised brushes were characterised using atomic force microscopy, attenuated total reflectance spectroscopy and electrochemical impedance spectroscopy. Once characterised, the polymer brushes were applied to the quantitative detection of target DNA using an enzyme labelled reporter DNA probe in a sandwich-type format. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1155 / 1158

页数：4

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