Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser

被引：21

作者：

Stagno, Jason R. ^{[1
]}

Bhandari, Yuba R. ^{[1
]}

Conrad, Chelsie E. ^{[1
]}

Liu, Yu ^{[1
]}

Wang, Yun-Xing ^{[1
]}

机构：

[1] NCI, Struct Biophys Lab, Frederick, MD 21701 USA

来源：

FEBS JOURNAL | 2017年 / 284卷 / 20期

关键词：

adenine riboswitch; diffusive mixing; lattice conversion; phase transition; reaction intermediate; RNA; serial femtosecond crystallography; time-resolved crystallography; X-ray free-electron laser; PHOTOACTIVE YELLOW PROTEIN; APTAMER DOMAIN; LIGAND-BINDING; STRUCTURAL INSIGHTS; LYSINE RIBOSWITCH; GENETIC-CONTROL; MESSENGER-RNA; RESOLUTION; MECHANISM; RECOGNITION;

D O I：

10.1111/febs.14110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular 'switch'.

引用

页码：3374 / 3380

页数：7

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