Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi

被引：11
作者：
Stevens, MK
Hassett, DJ
Radolf, JD
Hansen, EJ
机构：
[1] UNIV TEXAS, SW MED CTR, DEPT MICROBIOL, DALLAS, TX 75235 USA
[2] UNIV TEXAS, SW MED CTR, DEPT INTERNAL MED, DALLAS, TX 75235 USA
[3] UNIV CINCINNATI, DEPT MOL GENET BIOCHEM & MICROBIOL, CINCINNATI, OH 45267 USA
来源：
GENE | 1996年 / 183卷 / 1-2期
关键词：
metalloenzyme; sodC;
D O I：
10.1016/S0378-1119(96)00417-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.
引用
收藏
页码：35 / 40
页数：6
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