Serotyping is the foundation of pathogenic Escherichia coli diagnostics; however, few laboratories have this capacity. We developed a molecular serotyping protocol that targets, genetically, the same somatic and flagellar antigens of E. coli O26:H11 used in traditional serotyping. It correctly serotypes strains untypeable by traditional methods, affording primary laboratories serotyping capabilities.
机构:
USDA, Natl Anim Dis Ctr, Agr Res Serv, Preharvest Food Safety & Enter Dis Res Unit, Ames, IA 50010 USAUSDA, Natl Anim Dis Ctr, Agr Res Serv, Preharvest Food Safety & Enter Dis Res Unit, Ames, IA 50010 USA
机构:
USDA, Natl Anim Dis Ctr, Agr Res Serv, Preharvest Food Safety & Enter Dis Res Unit, Ames, IA 50010 USAUSDA, Natl Anim Dis Ctr, Agr Res Serv, Preharvest Food Safety & Enter Dis Res Unit, Ames, IA 50010 USA