Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5

被引：48

作者：

Kalb, Suzanne R. ^{[1
]}

Baudys, Jakub ^{[1
]}

Webb, Robert P. ^{[2
]}

Wright, Patrick ^{[2
]}

Smith, Theresa J. ^{[2
]}

Smith, Leonard A. ^{[3
]}

Fernandez, Rafael ^{[4
]}

Raphael, Brian H. ^{[5
]}

Maslanka, Susan E. ^{[5
]}

Pirkle, James L. ^{[1
]}

Barr, John R. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, Div Sci Lab, Atlanta, GA 30341 USA

[2] USAMRIID, Ft Detrick, MD 21702 USA

[3] Med Res & Mat Command MRMC, Off Chief Scientist, Ft Detrick, MD 21702 USA

[4] Univ Nacl Cuyo, Area Microbiol, RA-5500 Mendoza, Argentina

[5] Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, Enter Dis Lab Branch, Atlanta, GA 30329 USA

来源：

FEBS LETTERS | 2012年 / 586卷 / 02期

关键词：

Botulinum neurotoxin; BoNT/F; Synaptobrevin-2; Mass spectrometry; CLOSTRIDIAL NEUROTOXINS; SUBSTRATE RECOGNITION; MASS-SPECTROMETRY; SEROTYPE-A; SNAP-25; VAMP/SYNAPTOBREVIN; DIFFERENTIATION; PROTEOLYSIS; TETANUS; RELEASE;

D O I：

10.1016/j.febslet.2011.11.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Botulinum neurotoxins (BoNTs) cause botulism by cleaving proteins necessary for nerve transmission. There are seven serotypes of BoNT, A-G, characterized by their response to antisera. Many serotypes are further distinguished into differing subtypes based on amino acid sequence, some of which result in functional differences. Our laboratory previously reported that all tested subtypes within each serotype have the same site of enzymatic activity. Recently, three new subtypes of BoNT/F; /F3, /F4, and /F5, were reported. Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between 54L and 55E. This is the first report of cleavage of synaptobrevin-2 in this location.

引用

页码：109 / 115

页数：7

共 24 条

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