Complex regulation of Calbindin-D9k in the mouse placenta and extra-embryonic membrane during mid- and late pregnancy

被引：21

作者：

An, BS

Choi, KC

Lee, GS

Leung, PCK

Jeung, EB ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Vet Med, Lab Vet Biochem & Mol Biol, Cheongju 361763, Chungbuk, South Korea

[2] Univ British Columbia, BC Res Inst Childrens & Womens Hlth, Dept Obstet & Gynecol, Vancouver, BC V6H 3V5, Canada

[3] Seoul Natl Univ, Xenotransplantat Res Ctr, Seoul, South Korea

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2004年 / 214卷 / 1-2期

关键词：

Calbindin-D-9k; mouse placenta; extra-embryonic membrane;

D O I：

10.1016/j.mce.2003.11.029

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Calbindin-D-9k (CaBP-9k) is a cytosolic calcium-binding protein mainly expressed in the duodenum, uterus and placenta, however, the role of CaBP-9k in the regulation of fetal growth remains to be elucidated. The present study was performed to investigate the expression pattern and regulation of CaBP-9k by antagonists of steroid hormones related with steroid hormone receptors during mid- and late pregnancy in mouse placenta and extra-embryonic membrane. The expression level of CaBP-9k increased in the placenta, while it decreased in the extra-embryonic membrane during pregnancy. The mRNA expression levels of estrogen receptor alpha (ERalpha) and progesterone receptor (PR) appeared to increase in both placenta and extra-embryonic membrane during pregnancy, suggesting that the ER and PR mRNA and protein expressions of placental CaBP-9k are positively correlated, but expressions of extra-embryonic membrane CaBP-9k are reversely correlated with ERalpha and PR mRNA levels. In addition, the present study indicates that the expressions of CaBP-9k mRNA and protein are differentially up- or down-regulated by antagonists of estrogen (E2) and progesterone (P4) in mouse placenta and extra-embryonic membranes, which suggests that E2 and P4 may be dominant factors in the regulation of the CaBP-9k. In particular, RU486, an antagonist of P4, down-regulated the mRNA and protein levels of placental CaBP-9k, whereas it up-regulated the protein level of extra-embryonic membrane CaBP-9k. In conclusion, we demonstrated that the CaBP-9k is distinctly regulated in the mouse placenta and extra-embryonic membrane, probably via sex steroid hormones (E2 and P4) and their receptors through a complex pathway. Extended studies are needed to verify relevant factors to regulate CaBP-9k gene and to provide further insight into roles of CaBP-9k gene in these tissues for the control of reproductive functions. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：39 / 52

页数：14

共 47 条

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