An upstream repressor element plays a role in Igf2 imprinting

被引：79
作者：
Eden, S
Constancia, M
Hashimshony, T
Dean, W
Goldstein, B
Johnson, AC
Keshet, I
Reik, W
Cedar, H
机构：
[1] Hadassah Univ Hosp, Dept Cellular Biochem, IL-91120 Jerusalem, Israel
[2] Babraham Inst, Inst Grassland & Anim Prod, Cambridge CB2 4AT, England
[3] NCI, Canc Res Ctr, NIH, Bethesda, MD 20892 USA
来源：
EMBO JOURNAL | 2001年 / 20卷 / 13期
关键词：
GCF; imprinting; methylation;
D O I：
10.1093/emboj/20.13.3518
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele, We have identified a repressor element within this sequence and shown that repression is probably mediated through a transacting factor, GCF2, DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.
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页码：3518 / 3525
页数：8
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