A technique for the rapid extraction of microalgal DNA from single live and preserved cells

被引：121

作者：

Richlen, ML ^{[1
]}

Barber, PH ^{[1
]}

机构：

[1] Boston Univ, Marine Program, Dept Biol, Boston, MA 02215 USA

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

dinoflagellates; DNA extraction; Gambierdiscus; harmful algal blooms; single cell sequencing;

D O I：

10.1111/j.1471-8286.2005.01032.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular methods are increasingly being used in the study of harmful microalgae; however, DNA extraction techniques have imposed limitations on the species and questions studied, with research primarily restricted to cultured specimens. Here we describe a simple method that merges two existing techniques for DNA extraction from live and preserved single dinoflagellate cells. DNA was successfully isolated from live single cells of Gambierdiscus toxicus Adachi et Fukuyo,1979 and cells preserved using formalin/methanol fixation. This method supplements existing techniques and expands the scope of genetics studies conducted on dinoflagellates to include routine molecular analysis of single cells isolated from field samples.

引用

页码：688 / 691

页数：4

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