The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence

被引：24

作者：

Reffuveille, Fany ^{[1
]}

Serror, Pascale ^{[2
,3
]}

Chevalier, Sylvie ^{[4
]}

Budin-Verneuil, Aurelie ^{[1
]}

Ladjouzi, Rabia ^{[1
]}

Bernay, Benoit ^{[5
]}

Auffray, Yanick ^{[1
]}

Rince, Alain ^{[1
]}

机构：

[1] Univ Caen, USC INRA 2017, EA956, F-14032 Caen, France

[2] INRA, Micalis UMR 1319, F-78350 Jouy En Josas, France

[3] AgroParisTech, UMR Micalis, F-78350 Jouy En Josas, France

[4] Univ Rouen, Lab Microbiol Froid Signaux & Microenvironm, UPRES EA4312, F-76821 Mont St Aignan, France

[5] Univ Caen, Plateforme Proteogen IFR ICORE 146, F-14032 Caen, France

来源：

MICROBIOLOGY-SGM | 2012年 / 158卷

关键词：

OXIDATIVE STRESS; IN-VITRO; STREPTOCOCCUS-PNEUMONIAE; LIPID MODIFICATION; PROTEIN SECRETION; BACILLUS-SUBTILIS; LIPOPROTEIN; GROWTH; PRELIPOPROTEINS; TLR2;

D O I：

10.1099/mic.0.055319-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn2+ or under oxidative stress in vitro, and significantly decreased virulence.

引用

页码：816 / 825

页数：10

共 48 条

[1] [Anonymous], 1989, Molecular Cloning: A Laboratory
[2] Inactivation of lgt allows systematic characterization of lipoproteins from Listeria monocytogenes
Baumgaertner, Maja
Kaerst, Uwe
Gerstel, Birgit
Loessner, Martin
Wehland, Juergen
Jaensch, Lothar
[J]. JOURNAL OF BACTERIOLOGY, 2007, 189 (02) : 313 - 324
[3] SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS
BEAUCHAM.C
FRIDOVIC.I
[J]. ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) : 276 - &
[4] Glycerol Is Metabolized in a Complex and Strain-Dependent Manner in Enterococcus faecalis
Bizzini, Alain
Zhao, Chen
Budin-Verneuil, Aurelie
Sauvageot, Nicolas
Giard, Jean-Christophe
Auffray, Yanick
Hartke, Axel
[J]. JOURNAL OF BACTERIOLOGY, 2010, 192 (03) : 779 - 785
[5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6] Impact of Igt mutation on lipoprotein biosynthesis and in vitro phenotypes of Streptococcus agalactiae
Bray, Beverley A.
Sutcliffe, Iain C.
Harrington, Dean J.
[J]. MICROBIOLOGY-SGM, 2009, 155 : 1451 - 1458
[7] Enterococcal sex pheromone precursors are part of signal sequences for surface lipoproteins
Clewell, DB
An, FY
Flannagan, SE
Antiporta, M
Dunny, GM
[J]. MOLECULAR MICROBIOLOGY, 2000, 35 (01) : 246 - 247
[8] Surface-associated lipoprotein PpmA of Streptococcus pneumoniae is involved in colonization in a strain-specific manner
Cron, L. E.
Bootsma, H. J.
Noske, N.
Burghout, P.
Hammerschmidt, S.
Hermans, P. W. M.
[J]. MICROBIOLOGY-SGM, 2009, 155 : 2401 - 2410
[9] Mutation of the maturase lipoprotein attenuates the virulence of Streptococcus equi to a greater extent than does loss of general lipoprotein lipidation
Hamilton, Andrea
Robinson, Carl
Sutcliffe, Iain C.
Slater, Josh
Maskell, Duncan J.
Davis-Poynter, Nick
Smith, Ken
Waller, Andrew
Harrington, Dean J.
[J]. INFECTION AND IMMUNITY, 2006, 74 (12) : 6907 - 6919
[10] The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall
Hancock, LE
Gilmore, MS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) : 1574 - 1579

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