Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics

被引：380

作者：

Baerenfaller, Katja ^{[1
,2
]}

Grossmann, Jonas ^{[1
,2
,3
]}

Grobei, Monica A. ^{[2
,4
]}

Hull, Roger ^{[5
]}

Hirsch-Hoffmann, Matthias ^{[1
]}

Yalovsky, Shaul ^{[6
]}

Zimmermann, Philip ^{[1
]}

Grossniklaus, Ueli ^{[2
,4
]}

Gruissem, Wilhelm ^{[1
,2
,3
]}

Baginsky, Sacha ^{[1
,2
]}

机构：

[1] ETH, Swiss Fed Inst Technol, Inst Plant Sci, CH-8092 Zurich, Switzerland

[2] Univ Zurich, Ctr Model Organism Proteomes, CH-8057 Zurich, Switzerland

[3] Funct Genom Ctr Zurich, CH-8057 Zurich, Switzerland

[4] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland

[5] Univ Manchester, Fac Life Sci, Manchester M13 9PL, Lancs, England

[6] Tel Aviv Univ, Dept Plant Sci, IL-69978 Tel Aviv, Israel

来源：

SCIENCE | 2008年 / 320卷 / 5878期

关键词：

D O I：

10.1126/science.1157956

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have assembled a proteome map for Arabidopsis thaliana from high-density, organ-specific proteome catalogs that we generated for different organs, developmental stages, and undifferentiated cultured cells. We matched 86,456 unique peptides to 13,029 proteins and provide expression evidence for 57 gene models that are not represented in the TAIR7 protein database. Analysis of the proteome identified organ-specific biomarkers and allowed us to compile an organ-specific set of proteotypic peptides for 4105 proteins to facilitate targeted quantitative proteomics surveys. Quantitative information for the identified proteins was used to establish correlations between transcript and protein accumulation in different plant organs. The Arabidopsis proteome map provides information about genome activity and proteome assembly and is available as a resource for plant systems biology.

引用

页码：938 / 941

页数：4

共 27 条

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