Cloning and sequencing of β-mannosidase gene from Aspergillus aculeatus no. F-50

被引：19
作者：
Takada, G
Kawaguchi, T
Kaga, T
Sumitani, J
Arai, M [1 ]
机构：
[1] Univ Osaka Prefecture, Dept Appl Biol Chem, Sakai, Osaka 5998531, Japan
[2] Univ Osaka Prefecture, Res Inst Adv Sci & Technol, Sakai, Osaka 5998531, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 01期
关键词：
Aspergillus aculeatus; beta-mannosidase; beta-galactosidase; beta-guclonidase;
D O I：
10.1271/bbb.63.206
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The manB gene, coding for a unique beta-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli beta-galactosidase and the human beta-gluclonidase was conserved. This is the first fungal gene that encodes MANB.
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页码：206 / 209
页数：4
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