Cloning and sequencing of β-mannosidase gene from Aspergillus aculeatus no. F-50

被引：19

作者：

Takada, G

Kawaguchi, T

Kaga, T

Sumitani, J

Arai, M ^{[1
]}

机构：

[1] Univ Osaka Prefecture, Dept Appl Biol Chem, Sakai, Osaka 5998531, Japan

[2] Univ Osaka Prefecture, Res Inst Adv Sci & Technol, Sakai, Osaka 5998531, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 01期

关键词：

Aspergillus aculeatus; beta-mannosidase; beta-galactosidase; beta-guclonidase;

D O I：

10.1271/bbb.63.206

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The manB gene, coding for a unique beta-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli beta-galactosidase and the human beta-gluclonidase was conserved. This is the first fungal gene that encodes MANB.

引用

页码：206 / 209

页数：4

共 9 条

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