Determination of Catechol by a Novel Laccase Biosensor Based on Zinc-Oxide Sol-Gel

被引：19
作者：
Qu, Jianying [1 ]
Lou, Tongfang [1 ]
Wang, Yong [1 ]
Dong, Ying [1 ]
Xing, Huanhuan [1 ]
机构：
[1] Henan Univ, Inst Environm & Analyt Sci, Coll Chem & Chem Engn, Kaifeng 475001, Henan, Peoples R China
来源：
ANALYTICAL LETTERS | 2015年 / 48卷 / 12期
关键词：
ZnO sol-gel; Catechol; Laccase; Biosensor; TYROSINASE BIOSENSOR; PHENOLIC-COMPOUNDS; BIOFUEL CELL; NANOPARTICLES; PERFORMANCE; WATER; IMMOBILIZATION;
D O I：
10.1080/00032719.2014.1003427
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A biosensor was fabricated by incorporating laccase in a ZnO sol-gel with chitosan as a matrix for the determination of catechol. The ZnO nanoparticles were characterized by X-ray diffraction and atomic force microscopy. The conductivity of the chitosan/ZnO/glassy carbon electrode film was investigated by alternating current impedance. The biosensor was employed to monitor the reduction of catechol, and the peak current increased linearly with concentration between 1.0x10(-6) and 1.0x10(-4)mole per liter with a limit of detection of 2.9x10(-7)mole per liter. The laccase biosensor exhibited good stability, reproducibility, and some selectivity.
引用
收藏
页码：1842 / 1853
页数：12
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