Cisplatin-loaded gelatin-poly(acrylic acid) nanoparticles: Synthesis, antitumor efficiency in vivo and penetration in tumors

被引:75
作者
Ding, Dan
Zhu, Zhenshu
Liu, Qin [2 ,3 ]
Wang, Jing
Hu, Yong
Jiang, Xiqun [1 ]
Liu, Baorui [2 ,3 ]
机构
[1] Nanjing Univ, Coll Chem & Chem Engn, Lab Mesoscop Chem, Dept Polymer Sci & Engn, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Ctr Comprehens Canc, Drum Tower Hosp, Sch Med, Nanjing 210093, Peoples R China
[3] Nanjing Univ, Clin Canc Inst, Nanjing 210093, Peoples R China
基金
中国国家自然科学基金;
关键词
Polymer nanoparticles; Drug delivery; Antitumor; Cisplatin; Gelatin; GLYCOL CHITOSAN NANOPARTICLES; METAL COMPLEX MICELLE; HERPES-SIMPLEX-VIRUS; DRUG-DELIVERY; POLYMERIC MICELLES; EFFICACY; SYSTEM; BIODISTRIBUTION; NANOTECHNOLOGY; PERMEABILITY;
D O I
10.1016/j.ejpb.2011.01.008
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Cisplatin (CDDP)-loaded gelatin-poly(acrylic acid) (GEL-PAA) nanoparticles were successfully prepared by polymerizing acrylic acid in the presence of gelatin in aqueous solution followed by incorporating CDDP into the formed GEL-PAA nanoparticles through polymer-metal complex formation of CDDP with carboxylic groups in the nanoparticles. The obtained nanoparticles had a spherical shape, with a mean size of about 100 nm, and high drug payload as well as stability. It is found that CDDP can be released from the nanoparticles in a sustained manner with a small initial burst release. In vitro cytotoxicity revealed that CDDP-loaded nanoparticles had similar cytotoxicity to free CDDP after 48 h co-incubation with human colorectal cancer cell line LoVo. In vivo antitumor activity indicated that the nanoparticle formulation was superior in anticancer effect to free CDDP on murine hepatic H22 tumor-bearing mice model through intraperitoneal (i.p.) administration and displayed a dose-dependent antitumor efficacy. Further, the penetration examination of the nanoparticles through tumor tissue revealed that the CDDP-loaded GEL-PAA nanoparticles could only affect the cells near the tumor vasculature after they entered into the tumor tissue. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:142 / 149
页数:8
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