Approaches to detection of distantly related proteins by database searches

被引：0

作者：

Cattell, K

Koop, B

Olafson, RS

Fellows, M

Bailey, I

Olafson, RW

Upton, C

机构：

[1] UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,VICTORIA,BC V8W 3P6,CANADA

[2] BRITISH COLUMBIA SYST CORP,VICTORIA,BC,CANADA

来源：

BIOTECHNIQUES | 1996年 / 21卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The searching of protein databases as a method of identifying newly sequenced genes is commonplace in molecular biology laboratories. However, it is a procedure that is not usually formally taught to students, and method cookbooks discuss it only briefly. This article uses a single family of highly diverged uracil-DNA glycosylases, which fall into two distinct groups, to highlight some of the difficulties associated with identification of such proteins by database searching.

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页码：1118 / &

页数：6

相关论文

共 14 条

[1] PROTEIN DATABASE SEARCHES FOR MULTIPLE ALIGNMENTS [J].

ALTSCHUL, SF ;

LIPMAN, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (14) :5509-5513

[2]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[3]

ATWELL R, 1995, BIOTECHNIQUES, V19, P966

[4] PROSITE - A DICTIONARY OF SITES AND PATTERNS IN PROTEINS [J].

BAIROCH, A .

NUCLEIC ACIDS RESEARCH, 1991, 19 :2241-2245

[5] PROTEIN FAMILY CLASSIFICATION BASED ON SEARCHING A DATABASE OF BLOCKS [J].

HENIKOFF, S ;

HENIKOFF, JG .

GENOMICS, 1994, 19 (01) :97-107

[6] CRYSTAL-STRUCTURE AND MUTATIONAL ANALYSIS OF HUMAN URACIL-DNA GLYCOSYLASE - STRUCTURAL BASIS FOR SPECIFICITY AND CATALYSIS [J].

MOL, CD ;

ARVAI, AS ;

SLUPPHAUG, G ;

KAVLI, B ;

ALSETH, I ;

KROKAN, HE ;

TAINER, JA .

CELL, 1995, 80 (06) :869-878

[7] A GENERAL METHOD APPLICABLE TO SEARCH FOR SIMILARITIES IN AMINO ACID SEQUENCE OF 2 PROTEINS [J].

NEEDLEMAN, SB ;

WUNSCH, CD .

JOURNAL OF MOLECULAR BIOLOGY, 1970, 48 (03) :443-+

[8] COMPARISON OF METHODS FOR SEARCHING PROTEIN-SEQUENCE DATABASES [J].

PEARSON, WR .

PROTEIN SCIENCE, 1995, 4 (06) :1145-1160

[9] IMPROVED TOOLS FOR BIOLOGICAL SEQUENCE COMPARISON [J].

PEARSON, WR ;

LIPMAN, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2444-2448

[10] A POXVIRUS-ENCODED URACIL DNA GLYCOSYLASE IS ESSENTIAL FOR VIRUS VIABILITY [J].

STUART, DT ;

UPTON, C ;

HIGMAN, MA ;

NILES, EG ;

MCFADDEN, G .

JOURNAL OF VIROLOGY, 1993, 67 (05) :2503-2512