Effect of varicocele on sperm function and semen oxidative stress

被引：92

作者：

Blumer, Camile Garcia ^{[1
]}

Restelli, Adriana Ester ^{[1
]}

Del Giudice, Paula Toni ^{[1
]}

Soler, Thiesa Butterby ^{[1
]}

Fraietta, Renato ^{[1
]}

Nichi, Marcilio ^{[2
]}

Bertolla, Ricardo Pimenta ^{[1
]}

Cedenho, Agnaldo Pereira ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med, Sao Paulo, Brazil

来源：

BJU INTERNATIONAL | 2012年 / 109卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

varicocele; spermatozoa; oxidative stress; acrosome; DNA damage; mitochondria; metabolism; EJACULATED HUMAN SPERMATOZOA; NUCLEAR-DNA FRAGMENTATION; MALE-INFERTILITY; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; SUPEROXIDE-DISMUTASE; SEMINAL PLASMA; PART II; MEN; DAMAGE;

D O I：

10.1111/j.1464-410X.2011.10240.x

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE To assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity. PATIENTS AND METHODS In all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin). RESULTS The varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels. CONCLUSION Men with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged.

引用

页码：259 / 265

页数：7

共 49 条

[1] Role of oxidants in male infertility: rationale, significance, and treatment [J].

Agarwal, A ;

Saleh, RA .

UROLOGIC CLINICS OF NORTH AMERICA, 2002, 29 (04) :817-+

[2] Malondialdehyde level and total superoxide dismutase activity in seminal fluid from patients with varicocele [J].

Akyol, Ö ;

Özbek, E ;

Uz, E ;

Koçak, I .

CLINICAL AND EXPERIMENTAL MEDICINE, 2001, 1 (01) :67-68

[3]

ALVAREZ JG, 1987, J ANDROL, V8, P338

[4]

AMANN RP, 1989, J ANDROL, V10, P89

[5]

Ballester J, 2000, MOL REPROD DEV, V56, P207, DOI 10.1002/(SICI)1098-2795(200006)56:2<207::AID-MRD12>3.0.CO

[6]

2-4

[7]

Baumber J, 2000, J ANDROL, V21, P895

[8] Sperm nuclear DNA fragmentation in adolescents with varicocele [J].

Bertolla, RP ;

Cedenho, AP ;

Hassun, PA ;

Lima, SB ;

Ortiz, V ;

Srougi, M .

FERTILITY AND STERILITY, 2006, 85 (03) :625-628

[9]

BROWN JS, 1967, FERTIL STERIL, V18, P46

[10]

Chen SS, 2008, J UROLOGY, V179, P639, DOI 10.1016/j.juro.2007.09.039

← 1 2 3 4 5 →