Acute Hepatic Failure-Derived Bone Marrow Mesenchymal Stem Cells Express Hepatic Progenitor Cell Genes

被引：6

作者：

Li, Jun ^{[1
]}

Wu, Wei ^{[1
]}

Xin, Jiaojiao ^{[1
]}

Guo, Jing ^{[1
]}

Jiang, Longyan ^{[1
]}

Tao, Ran ^{[1
]}

Cao, Hongcui ^{[1
]}

Hong, Xutao ^{[2
]}

Li, Lanjuan ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Calif Int Nanosyst Inst, Hangzhou, Zhejiang, Peoples R China

来源：

CELLS TISSUES ORGANS | 2011年 / 194卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Bone marrow stromal cell; Gene transcription; Hepatology; Stem cells; Hepatic cells; ADULT-RAT LIVER; STROMAL CELLS; DIFFERENTIATION; TRANSPLANTATION; HEPATOCYTES; TISSUE; ESTABLISHMENT; REPLACEMENT; THERAPY; DISEASE;

D O I：

10.1159/000322604

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Hepatic progenitor cell (HPC) transplantation is a promising alternative to liver transplantation for patients with end-stage liver disease. However, the precise origin of HPCs is unclear. This study aimed to determine whether bone marrow mesenchymal stem cells (BMSCs) isolated from rats in acute hepatic failure (AHF) possess hepatic potential and/or characteristics. BMSCs were isolated from normal rats as well as rats in which AHF was induced by D-galactosamine. HPCs and primary hepatocytes were isolated from normal rats for comparison. The Affymetrix GeneChip Rat Genome 230 2.0 Array was used to perform transcriptome profiling of the AHF-derived BMSCs and HPCs. The results showed that AHF-derived BMSCs had a gene expression profile significantly different from that of control BMSCs. More than 87.7% of the genes/probe sets that were upregulated more than 2-fold in AHF-derived BMSCs were expressed by HPCs, including 12 genes involved in liver development, early hepatocyte differentiation and hepatocyte metabolism. Confirmatory quantitative RT-PCR analysis yielded similar results. In addition, 940 probe sets/genes were expressed in both AHF-derived BMSCs and HPCs but were absent in control cells. Compared to the control cells, AHF-derived BMSCs shared more commonly expressed genes with HPCs. AHF-derived BMSCs have a hepatic transcriptional profile and express many of the same genes expressed by HPCs, strongly suggesting that BMSCs may be a resource for hepatocyte regeneration, and further confirming their potential as a strong source of hepatocyte regeneration during severe hepatic damage. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：371 / 381

页数：11

共 43 条

[31] Bone marrow as a potential source of hepatic oval cells [J].

Petersen, BE ;

Bowen, WC ;

Patrene, KD ;

Mars, WM ;

Sullivan, AK ;

Murase, N ;

Boggs, SS ;

Greenberger, JS ;

Goff, JP .

SCIENCE, 1999, 284 (5417) :1168-1170

[32] Differentiation potential of FDCPmix cells following injection into blastocysts [J].

Petrovic, S ;

Cross, M ;

Müller, AM .

CELLS TISSUES ORGANS, 2004, 178 (02) :78-86

[33] Hematopoietic mobilization in mice increases the presence of bone marrow-derived hepatocytes via in vivo cell fusion [J].

Quintana-Bustamante, O ;

Alvarez-Barrientos, A ;

Kofman, AV ;

Fabregat, I ;

Bueren, JA ;

Theise, ND ;

Segovia, JC .

HEPATOLOGY, 2006, 43 (01) :108-116

[34] Is it right to promote living donor liver transplantation for fulminant hepatic failure in pediatric recipients? [J].

Reding, R .

AMERICAN JOURNAL OF TRANSPLANTATION, 2005, 5 (07) :1587-1591

[35] Cellular therapies for liver replacement [J].

Selden, C ;

Hodgson, H .

TRANSPLANT IMMUNOLOGY, 2004, 12 (3-4) :273-288

[36] Practical Barriers to Delivering Autologous Bone Marrow Stem Cell Therapy as an Adjunct to Liver Resection [J].

Stutchfield, Benjamin M. ;

Rashid, Sameena ;

Forbes, Stuart J. ;

Wigmore, Stephen J. .

STEM CELLS AND DEVELOPMENT, 2010, 19 (02) :155-161

[37] Liver from bone marrow in humans [J].

Theise, ND ;

Nimmakayalu, M ;

Gardner, R ;

Illei, PB ;

Morgan, G ;

Teperman, L ;

Henegariu, O ;

Krause, DS .

HEPATOLOGY, 2000, 32 (01) :11-16

[38] Hematopoietic cells as hepatocyte stem cells: A critical review of the evidence [J].

Thorgeirsson, SS ;

Grisham, JW .

HEPATOLOGY, 2006, 43 (01) :2-8

[39] Bone Marrow-Derived Nonreactive Astrocytes in the Mouse Brain After Permanent Middle Cerebral Artery Occlusion [J].

Toth, Zsuzsanna E. ;

Leker, Ronen R. ;

Shahar, Tal ;

Bratincsak, Andras ;

Szalayova, Ildiko ;

Key, Sharon ;

Miklos Palkovits ;

Cassiani-Ingoni, Riccardo ;

Mezey, Eva .

STEM CELLS AND DEVELOPMENT, 2011, 20 (03) :539-546

[40] Transcriptomic fingerprinting of bone marrow-derived hepatic β2m-/Thy-1+ stem cells [J].

Wang, C ;

Chelly, MR ;

Chai, NN ;

Tan, YX ;

Hui, T ;

Li, HM ;

Farkas, DL ;

Demetriou, AA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 327 (01) :252-260

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