Global landscape of cell envelope protein complexes in Escherichia coli

被引：95

作者：

Babu, Mohan ^{[1
]}

Bundalovic-Torma, Cedoljub ^{[2
,3
]}

Calmettes, Charles ^{[3
,4
]}

Phanse, Sadhna ^{[1
,5
]}

Zhang, Qingzhou ^{[1
]}

Jiang, Yue ^{[2
]}

Minic, Zoran ^{[1
]}

Kim, Sunyoung ^{[1
]}

Mehla, Jitender ^{[6
]}

Gagarinova, Alla ^{[7
]}

Rodionova, Irina ^{[8
]}

Kumar, Ashwani ^{[9
]}

Guo, Hongbo ^{[5
]}

Kagan, Olga ^{[5
]}

Pogoutse, Oxana ^{[5
]}

Aoki, Hiroyuki ^{[1
]}

Deineko, Viktor ^{[1
]}

Caufield, J. Harry ^{[6
]}

Holtzapple, Erik ^{[7
,8
]}

Zhang, Zhongge ^{[8
]}

Vastermark, Ake ^{[8
]}

Pandya, Yogee ^{[5
]}

Lai, Christine Chieh-lin ^{[3
]}

El Bakkouri, Majida ^{[3
]}

Hooda, Yogesh ^{[3
]}

Shah, Megha ^{[3
]}

Burnside, Dan ^{[10
]}

Hooshyar, Mohsen ^{[10
]}

Vlasblom, James ^{[1
]}

Rajagopala, Sessandra V. ^{[11
]}

Golshani, Ashkan ^{[10
]}

Wuchty, Stefan ^{[12
,13
]}

Greenblatt, Jack F. ^{[5
,14
]}

Saier, Milton ^{[8
]}

Uetz, Peter ^{[6
]}

Moraes, Trevor F. ^{[3
]}

Parkinson, John ^{[2
,3
,14
]}

Emili, Andrew ^{[5
,14
,15
]}

机构：

[1] Univ Regina, Dept Biochem, Regina, SK, Canada

[2] Hosp Sick Children, Toronto, ON, Canada

[3] Univ Toronto, Dept Biochem, Toronto, ON, Canada

[4] INRS Inst Armand Frappier, Laval, PQ, Canada

[5] Univ Toronto, Donnelly Ctr, Toronto, ON, Canada

[6] Virginia Commonwealth Univ, Richmond, VA USA

[7] Univ Saskatchewan, Dept Biochem, Saskatoon, SK, Canada

[8] Univ Calif San Diego, Dept Mol Biol, La Jolla, CA USA

[9] Univ Regina, Dept Comp Sci, Regina, SK, Canada

[10] Carleton Univ, Dept Biol, Ottawa, ON, Canada

[11] J Craig Venter Inst, Rockville, MD USA

[12] Univ Miami, Dept Comp Sci, Coral Gables, FL 33124 USA

[13] Univ Miami, Dept Biol, Coral Gables, FL 33124 USA

[14] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[15] Boston Univ, Dept Biol & Biochem, Boston, MA 02215 USA

来源：

NATURE BIOTECHNOLOGY | 2018年 / 36卷 / 01期

基金：

加拿大自然科学与工程研究理事会; 美国国家卫生研究院; 加拿大健康研究院; 加拿大创新基金会;

关键词：

OUTER-MEMBRANE; TRANSPORT-SYSTEM; CARDIOLIPIN; PREDICTION; DISCOVERY; NETWORK; IDENTIFICATION; TRANSLOCATION; IONIZATION; EXPRESSION;

D O I：

10.1038/nbt.4024

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial cell envelope protein (CEP) complexes mediate a range of processes, including membrane assembly, antibiotic resistance and metabolic coordination. However, only limited characterization of relevant macromolecules has been reported to date. Here we present a proteomic survey of 1,347 CEPs encompassing 90% inner- and outer-membrane and periplasmic proteins of Escherichia coli. After extraction with non-denaturing detergents, we affinity-purified 785 endogenously tagged CEPs and identified stably associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising 77% of targeted CEPs, revealed many previously uncharacterized heteromeric complexes. We found that the secretion of autotransporters requires translocation and the assembly module TamB to nucleate proper folding from periplasm to cell surface through a cooperative mechanism involving the beta-barrel assembly machinery. We also establish that an ABC transporter of unknown function, YadH, together with the Mla system preserves outer membrane lipid asymmetry. This E. coli CEP 'interactome' provides insights into the functional landscape governing CE systems essential to bacterial growth, metabolism and drug resistance.

引用

页码：103 / +

页数：13

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