Influenza M1 VLPs containing neuraminidase induce heterosubtypic cross-protection

被引：59
作者：
Quan, Fu-Shi [3 ]
Kim, Min-Chul [4 ,5 ]
Lee, Byeong-Jae [4 ,5 ]
Song, Jae-Min [6 ]
Compans, Richard W. [4 ,5 ]
Kang, Sang-Moo [1 ,2 ]
机构：
[1] Georgia State Univ, Ctr Inflammat Immun & Infect, Atlanta, GA 30303 USA
[2] Georgia State Univ, Dept Biol, Atlanta, GA 30303 USA
[3] Kyung Hee Univ, Dept Med Zool, Sch Med, Seoul 130701, South Korea
[4] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA
[5] Emory Univ, Sch Med, Emory Vaccine Ctr, Atlanta, GA 30322 USA
[6] Sungshin Womens Univ, Dept Global Med Sci, Seoul, South Korea
来源：
VIROLOGY | 2012年 / 430卷 / 02期
关键词：
Influenza; Virus-like particles; Neuraminidase; Protection; VIRUS-LIKE PARTICLES; INTRAVIRIONIC ANTIGENIC-COMPETITION; EXPRESSING H5 HEMAGGLUTININ; BROADENED IMMUNE-RESPONSE; A VIRUS; AVIAN INFLUENZA; LETHAL INFLUENZA; DENDRITIC CELLS; VIRAL NEURAMINIDASE; ANTIBODY-RESPONSE;
D O I：
10.1016/j.virol.2012.05.006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Influenza virus like particles (VLPs) containing hemagglutinin were previously demonstrated to induce protection against the homologous strains. However, little information is available on the protective role of neuraminidase (NA), the second major glycoprotein. In this study, we developed VLPs (NA VLPs) containing NA and M1 derived from A/PR/8/34 (H1N1) influenza virus, and investigated their ability to induce protective immunity. Intranasal immunization with NA VLPs induced serum antibody responses to H1N1 and H3N2 influenza A viruses as well as significant neuraminidase inhibition activity. Importantly, mice immunized with NA VLPs were 100% protected against lethal infection by the homologous A/PR/8/34 (H1N1) as well as heterosubtypic A/Philippines/82 (H3N2) virus, although body weight loss was observed after lethal challenge with heterosubtypic H3N2 virus. The present study therefore provides evidence that influenza VLPs containing M1 and NA are capable of inducing immunity to homologous as well as antigenically distinct influenza A virus strains. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：127 / 135
页数：9
相关论文
共 60 条
[21]   Changing perspective on immunization against influenza [J].
Johansson, Bert E. ;
Brett, Ian C. .
VACCINE, 2007, 25 (16) :3062-3065
[22]   MyD88 Plays an Essential Role in Inducing B Cells Capable of Differentiating into Antibody-Secreting Cells after Vaccination [J].
Kang, Sang-Moo ;
Yoo, Dae-Goon ;
Kim, Min-Chul ;
Song, Jae-Min ;
Park, Min-Kyung ;
Eunju, O. ;
Quan, Fu-Shi ;
Akira, Shizuo ;
Compans, Richard W. .
JOURNAL OF VIROLOGY, 2011, 85 (21) :11391-11400
[23]   Influenza vaccines based on virus-like particles [J].
Kang, Sang-Moo ;
Song, Jae-Min ;
Quan, Fu-Shi ;
Compans, Richard W. .
VIRUS RESEARCH, 2009, 143 (02) :140-146
[24]   Induction of Long-Term Protective Immune Responses by Influenza H5N1 Virus-Like Particles [J].
Kang, Sang-Moo ;
Yoo, Dae-Goon ;
Lipatov, Aleksandr S. ;
Song, Jae-Min ;
Davis, C. Todd ;
Quan, Fu-Shi ;
Chen, Li-Mei ;
Donis, Ruben O. ;
Compans, Richard W. .
PLOS ONE, 2009, 4 (03)
[25]   Modified HIV envelope proteins with enhanced binding to neutralizing monoclonal antibodies [J].
Kang, SM ;
Quan, FS ;
Huang, CZ ;
Guo, LH ;
Ye, L ;
Yang, CL ;
Compans, RW .
VIROLOGY, 2005, 331 (01) :20-32
[26]   EFFECT OF INFLUENZA ANTI-NEURAMINIDASE ANTIBODY ON VIRUS NEUTRALIZATION [J].
KASEL, JA ;
COUCH, RB ;
GERIN, JL ;
SCHULMAN, JL .
INFECTION AND IMMUNITY, 1973, 8 (01) :130-131
[27]   INDEPENDENT AND DISPARATE EVOLUTION IN NATURE OF INFLUENZA-A VIRUS HEMAGGLUTININ AND NEURAMINIDASE GLYCOPROTEINS [J].
KILBOURNE, ED ;
JOHANSSON, BE ;
GRAJOWER, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :786-790
[28]   Enhanced Memory Responses to Seasonal H1N1 Influenza Vaccination of the Skin with the Use of Vaccine-Coated Microneedles [J].
Kim, Yeu-Chun ;
Quan, Fu-Shi ;
Yoo, Dae-Goon ;
Compans, Richard W. ;
Kang, Sang-Moo ;
Prausnitz, Mark R. .
JOURNAL OF INFECTIOUS DISEASES, 2010, 201 (02) :190-198
[29]   Essential sequence of influenza neuraminidase DNA to provide protection against lethal viral infection [J].
Li, XZ ;
Fang, F ;
Song, YL ;
Yan, HL ;
Chang, HY ;
Sun, SH ;
Chen, Z .
DNA AND CELL BIOLOGY, 2006, 25 (04) :197-205
[30]   Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase [J].
Martinet, W ;
Saelens, X ;
Deroo, T ;
Neirynck, S ;
Contreras, R ;
Jou, WM ;
Fiers, W .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 247 (01) :332-338
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