Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

被引：17

作者：

Abou-El-Ardat, Khalil ^{[1
,2
]}

Monsieurs, Pieter ^{[1
]}

Anastasov, Natasa ^{[3
]}

Atkinson, Mike ^{[3
]}

Derradji, Hanane ^{[1
]}

De Meyer, Tim ^{[2
,4
]}

Bekaert, Sofie ^{[5
]}

Van Criekinge, Wim ^{[2
]}

Baatout, Sarah ^{[1
,2
]}

机构：

[1] Ctr Etud Energie Nucl SCK CEN, Radiobiol Unit, Studiectr Kernenergie, B-2400 Mol, Belgium

[2] Univ Ghent, Fac Biosci Engn, Dept Mol Biotechnol, B-9000 Ghent, Belgium

[3] Helmholtz Zentrum Munchen, Dept Radiat Sci, Munich, Germany

[4] Univ Ghent, Fac Biosci Engn, Dept Appl Math Biometr & Proc Control, B-9000 Ghent, Belgium

[5] Univ Ghent, Clin Res Ctr, Fac Med & Hlth Sci, B-9000 Ghent, Belgium

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2012年 / 731卷 / 1-2期

关键词：

RET/PTC translocation; Thyroid; Micro RNA; X-irradiation; Low doses; MACROPHAGE INHIBITORY CYTOKINE-1; PERIPHERAL-BLOOD LYMPHOCYTES; RECEPTOR TYROSINE KINASE; IONIZING-RADIATION; GENE-EXPRESSION; TUMOR-SUPPRESSOR; PAPILLARY CARCINOMAS; HUMAN FIBROBLASTS; TRANSGENIC MICE; POST-CHERNOBYL;

D O I：

10.1016/j.mrfmmm.2011.10.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The high doses of radiation received in the wake of the Chernobyl incident and the atomic bombing of Hiroshima and Nagasaki have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using Affymetrix microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids. This observation should be studied further as opposed to its effect on RET/PTC-positive thyroids which was subtle, anti-proliferative and system-dependent. (C) 2011 Elsevier By. All rights reserved.

引用

页码：27 / 40

页数：14

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